A gel protein capillary extraction apparatus is developed and demonstrated for its rapid and effective transfer of SDS-protein complexes from polyacrylamide gel to a fused-silica capillary. The small dimensions of capillary columns permit the application of high voltages for achieving rapid and effective transfer of gel proteins. Furthermore, the fused-silica capillaries are internally coated w...

Theory suggests that the effects of disadvantaged neighborhoods on child educational outcomes may depend on a family’s economic resources as well as the timing of neighborhood exposures during the course of child development. However, most previous research assumes that disadvantaged neighborhoods have the same effects on all children regardless of their family resources, and few prior studies ...

Theory suggests that neighborhood effects depend not only on where individuals live today, but also on where they lived in the past. Previous research, however, usually measures neighborhood context only once and does not account for length of residence, thereby understating the detrimental effects of long-term neighborhood disadvantage. This study investigates effects of duration of exposure t...

Deftection of oriented ftakeboards, random ftakeboards, and southern pine plywood was evaluated for small size bending specimens and concentrated loads applied to panels nailed on framing lumber. The ftakeboards con~ed a mixture of southern hardwoods and pine; the plywood was 3-ply l/2-inch and 4-ply ~-inch construction. Tests of both panel ~ons, all load levels and RH cycles showed plywood ben...

The analysis of similar earthquakes, such as events in a seismic sequence, is an effective tool with which to monitor and study source processes and to understand the mechanical and dynamic states of active fault systems. We are observing seismicity that is primarily concentrated in very limited regions along the 1980 Irpinia earthquake fault zone in Southern Italy, which is a complex system ch...

The effective and sustained delivery of DNA and siRNAs locally would increase the applicability of gene therapy in tissue regeneration and cancer therapy. One promising approach is to use hydrogel scaffolds to encapsulate and deliver nucleotides in the form of nanoparticles to the disease sites. However, this approach is currently limited by the inability to load concentrated and active gene de...

Dissolving microneedle patches offer promise as a simple, minimally invasive method of drug and vaccine delivery to the skin that avoids the need for hypodermic needles. However, it can be difficult to control the amount and localization of drug within microneedles. In this study, we developed novel microneedle designs to improve control of drug encapsulation and delivery using dissolving micro...

Casts of upper canine teeth of 15 species of microchiropteran bats were fixed securely into a testing machine and made to puncture the bloom side of an apple. The force necessary to break through the surface of the apple was regressed against both shape of the tip of the canine and the size of the animal. Sharper tips require less force to puncture than blunt ones. Results were also verified us...

A concentrated solution of purified enterokinase is conveniently prepared from the fluid contents of pigs' duodena by means of fractional precipitation with ammonium sulfate under the proper pH conditions.