NTERALLELIC complementation has been demonstrated for mutants at variIous loci (see review by YANOFSKY and ST. LAWRENCE 1960), including the hist-3 mutants of Neurospora (WEBBER 1959, 1960; CATCHESIDE 1960). Allelic mutants are tested in various combinations or in all possible combinations of pairs in heterokaryon tests, and the interaction matrix resulting from these tests is used to develop a...

We have conducted a genetic analysis of the region flanking the 68C glue gene cluster in Drosophila melanogaster by isolating lethal and semilethal mutations uncovered by deficiencies which span this region. Three different mutagens were used: ethyl methanesulfonate (EMS), ethyl nitrosourea (ENU) and diepoxybutane (DEB). In the region from 68A3 to 68C11, 64 lethal, semilethal, and visible mutat...

A novel approach for functional complementation of foreign genes in Saccharomyces cerevisiae is presented. This approach is based on the use of the widely available cognate gene plasmids (e.g. pRS416) of the European Functional Analysis Network (EUROFAN). The functional complementation of the human homolog of YOR159c (SME1 gene) shown here is the first demonstration of complementation using the...

Several recent studies have used quantitative complementation tests to identify relatively short chromosome regions that contain genes that influence life span and to screen for candidate life-span genes in flies. The methodology and logic of quantitative complementation tests are described. Arguments are presented that suggest that these tests may be misleading because there is a substantial, ...

Lymphocytes from tx-mutant mice have a definite and consistent pattern of reactivity in MLR. Cells from t-mutant mice within a complementation group usually fail to stimulate each other, while cells from mutants in different complementation groups stimulate each other strongly. This indicates that tx-mutant types are associated with certain H-2 haplotypes, and that members of any give complemen...

The 73AD salivary chromosome region of Drosophila melanogaster was subjected to mutational analysis in order to (1) generate a collection of chromosome breakpoints that would allow a correlation between the genetic, cytological and molecular maps of the region and (2) define the number and gross organization of complementation groups within this interval. Eighteen complementation groups were de...

The development of grammatical forms and synchronic effects has captured a considerable interest researchers across the globe. research this phenomenon is done to understand how certain linguistic arise interact with other forms. Literature also discloses that number studies addressed grammaticalisation prepositions, which somehow resulted in categorial conflict. However, there dearth literatur...

AIM To investigate genetic heterogeneity within the cblA class of inborn error of cobalamin metabolism. CONTEXT The cblA disorder is characterised by vitamin B12 (cobalamin) responsive methylmalonic aciduria and deficient synthesis of adenosylcobalamin, required for activity of the mitochondrial enzyme methylmalonyl CoA mutase. The cblA gene has not been identified or cloned. We have previous...

Many proteins can have their peptide backbone cut by proteolytic or genetic means, yet the two fragments can associate to make an active heterodimer. This ‘‘monomer-to-heterodimer conversion’’ is referred to as protein fragment complementation (PFC). Such complementation is the reverse of evolutionary processes in which domains are recruited and fused at the genetic level. Classic examples of p...

The genetic relationships between the multiple variants of mucolipidosis II (I-cell disease) and mucolipidosis III (pseudo-Hurler polydystrophy) were investigated with a sensitive genetic complementation analysis procedure. These clinically distinct disorders have defects in the synthesis of a recognition marker necessary for the intracellular transport of acid hydrolases into lysosomes. Both d...