The colony forming units in culture (cfu-c) in bone marrowcells, which were suppressed by immunosuppressive factor obtained from the serum of sarcoma 180 tumor bearing mouse, was restored to normal level by the addition of FR-900490in vitro. Furthermore, in mitomycin C (MMC)-treated mice the subsequent administration of FR-900490caused a significant increase of cfu-c in bone marrow cells depres...

The effects of two different potent inhibitors of protein kinase C, 1-(5-isoquinolinylsulfonyl)-2-methylpiperazine (H-7) and staurosporine on human myeloid (CFU-C) and late erythroid progenitor cells (CFU-E) were studied using an in vitro clonal assay. Our objective was to determine whether protein kinase C has a role in signal transduction related to proliferation of these committed progenitor...

The best practice for cleansing urinary drainage bags (leg and bed) in a hospital setting can be translated for use by clients for self-care within the home setting. Common household bleach containing 5.25% sodium hypochlorite, diluted in a 1:10 ratio with tap water, is a safe and effective method to reduce colony-forming units per milliliter (CFU/ml) to a study-defined acceptable range of 100 ...

Two classes of human marrow megakaryocyte progenitor cells are described. Colony-forming unit-megakaryocyte (CFU-MK)-derived colonies appeared in vitro after 12-day incubation; burst-forming unit-megakaryocyte (BFU-MK)-derived colonies appeared after 21 days. CFU-MK-derived colonies were primarily unifocal and composed of 11.6 +/- 1.2 cells/colony; BFU-MK-derived colonies were composed of 2.3 +...

An in vitro liquid suspension culture system was used to determine the role of cytokines in sustaining long-term human megakaryocytopoiesis. Bone marrow cells expressing CD34 but not HLA-DR (CD34+DR-) were used as the inoculum of cells to initiate long-term bone marrow cultures (LTBMC). CD34+DR- cells (5 x 10(3)/mL) initially contained 0.0 +/- 0.0 assayable colony-forming unit-megakaryocytes (C...

We studied the transport of nitrogen mustard (HN2) by mouse hemopoietic and transplantable myeloma (Adj. PC-5 and MOPC 460D) stem cells, using gradients of tempera ture and choline chloride. Bone marrow and myeloma cells were exposed to HN2 in culture at various temperatures, washed, and tested for colony-forming efficiency. The protective effect of lowered temperatures was similar for normal m...

We have investigated the role of host immunological factors in the formation of "tumor colonies" in the spleens of unirradiated C57BL/6 X C3Hf/Bi FI mice 9 days after i.v. injection of spleen cells from Friend virus (FV)-infected C3Hf/Bi donors. Pretreatment of hosts with antilymphocyte serum (ATS) increased the number of tumor colonies. Pretreatment with formalinized FV-infected cells had the ...

BACKGROUND Use of protective footwear before entering the intensive care units is enforced with the assumption that it lowers the incidence of bacterial floor colonization. The present study was carried out to find the efficacy of protective footwear on bacterial floor colonization. METHODS The study was carried out in the intensive care unit of a tertiary care hospital. The study was divided...

The effects of transforming growth factor flu or (TGF-/11 or -p2) on the in vitro proliferation and differentiation of normal and malignant human hematopoietic cells were studied. Both forms of TGF-fl suppressed both the normal cellular proliferation and colony formation induced by recombinant human interleukin-3 (IL-3) and granulocyte-macrophage colony-stimulating factor (GM-CSF). In the prese...

conclusion these data show that the identified gene encoded tet resistance gene as mfs–type multi-substrate efflux transporter, which contributes to intrinsic resistance on range of structurally unrelated compounds in salmonella species. background tetracyclines (tcs) are a type of broad-spectrum bacteriostatic antibiotics used for treatment of a wide variety of infections. these antibiotics ev...