Levels of haem synthesis achieved by foetal liver erythroblasts responding to erythropoietin in vitro are similar in dissociated cell cultures and in cultures of organized tissues. Erythroid colony-forming cells reach maximum numbers on the sixteenth day of gestation. Their presence in foetal liver is associated with the period of most rapid production of erythrocytes, and with in vitro sensiti...

The relationship between hematopoietic colony-forming stem cells and cells in the thymus and lymph nodes of unirradiated mice has been investigated using a chromosome-marker technique. It was found that a high proportion of cells in the thymus may belong to the same clone as normal hematopoietic colony-forming cells. It was also found that cells belonging to the same clone as colony-forming cel...

The epidermis and its related appendages such as the hair follicle constitute the epithelial compartment of the skin. The exact location and distribution of the keratinocyte colony-forming cells within the epidermis or its appendages are unknown. We report that in the rat vibrissa, keratinocyte colony-forming cells are highly clustered in the bulge-containing region. Approximately 95% of the to...

BACKGROUND Cytosine arabinoside (Ara-C) is an S-phase specific cytotoxic drug used in the treatment of malignancies. It is converted to Cytosine Arabinoside triphosphate (Ara-CTP) in the cell. Cytosine Arabinoside triphosphate, reversibly displaces deoxy cytidine triphosphate from DNA polymerase for incorporation into DNA. This process leads to cell death. OBJECTIVE To investigate the in vivo...

Human embryonic stem cell (hESC)-derived hematopoietic stem/progenitor cells hold tremendous potential as alternative cell sources for the treatment of various hematological diseases, drug discovery and toxicological screening. However, limited number of hematopoietic stem/progenitor cells generated from the differentiation of hESCs hinders their downstream applications. Here, we show that aryl...

AIMS A microbiological study of uncooked ready-to-eat organic vegetables was undertaken to determine the microbiological quality of these vegetables on retail sale in the UK. METHODS AND RESULTS Organic vegetables were collected and examined according to a standardized protocol. The majority (3185 of 3200; 99.5%) of samples were found to be of satisfactory/acceptable quality whilst only 15 (0...

The diagnosis of ASB is the most important step in managing ASB. As stated at the article the most important point for diagnosis is microbiological tests. In this context the number of specimens for culture is crucial. In guidelines ASB in men is defined as the isolation of ≥ 105 cfu/mL bacteria in a single cleancatch voided urine specimen. On the other hand in women, two consecutive urine spec...