the objective of this study was to evaluate the effect of the sto feeder layer on prepubertal rhode island red rooster sscs culture and proliferation in vitro. testis cells from 30 prepubertal rhode island red chicken (4-8 weeks of age), were individually separated and cultivated in the presence of  bfgf and lif growth factors on four well plates with two treatments and three replicats and five...

The effect of C. albicans infection on the production of haematopoietic precursor cells in the bone marrow of CBA/CaH and BALB/c mice was evaluated by assay of colony formation in vitro. In immunocompetent mice, neither systemic nor oral infection induced significant alterations in colony formation by bone marrow from the two mouse strains, and Candida infection did not alter the proportion of ...

We studied the effects ofIL4 on the spontaneous proliferation of chronic myelomonocytic leukemia (CMMoL) cells in vitro. ILA (100 U/ml) suppressed the spontaneous DNA synthesis by 50% in 5 of 8 cases examined. 1L4 (100 U/ml) also inhibited the spontaneous colony formation by CMMoL cells in a methylceliulose culture by 50-97% in all of the 10 cases in which spontaneous colonies were formed. This...

Purpose: To investigate the function and interaction of heat-shock cognate protein 70 (Hsc70) potassium voltage-gated channel subfamily A member 5 (KCNA5) in polycystic ovary syndrome (PCOS).Methods: Quantitative reverse transcription polymerase chain reaction (qRT-PCR) western blot were used to measure Hsc70 KCNA5 expression PCOS. The effect on granulosa cell apoptosis was determined by flow c...

introduction: the spermatogonial stem cells (sscs) form the basis of spermatogenesis. in vitro culture of germ cells is useful for the purification and increasing of sscs for transplantation. in the present study, we examined: in vitro the effects of co-culture with sertoli cells, gdnf, scf and gm-csf on efficiency of spermatogonial cells colony formation and spermatogenesis induction in the re...

the complex process of spermatogenesis is regulated by various factors. studies on spermatogonial stem cells (sccs) have provided very important tool to improve herd genetic and different field. 0.2 to 0.3 percent of total cells of seminiferous tubules is consist of spermatogonial stem cells. to investigate and biomanipulation of these cells, proliferation and viability rate of cells should be ...

This study aimed to investigate the effect of RNAi-mediated silencing of the Livin gene on biological properties of the colon cancer cell line LoVo. Interference vectors pSilencer4.1-Ll and pSilencer4.1-L2 targeting the Livin gene were constructed and transfected into LoVo cells. The expression of the Livin gene was determined by RT-PCR and Western blotting. The apoptosis, cell cycle, colony fo...

Surprisingly little is known about the role of host factors in regulating transposition, despite the potentially deleterious rearrangements caused by the movement of transposons. An extensive mutant screen was therefore conducted to identify Escherichia coli host factors that regulate transposition. An E. coli mutant library was screened using a papillation assay that allows detection of IS903 ...

Purpose: To determine the potential effect of Chikusetsu saponin IVA (CS-IVA) on benign prostatic hyperplasia (BPH), as well mechanism action.
 Methods: Benign epithelial cell response to treatment by CS-IVA was evaluated 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT), colony formation assay, and cycle analysis. Flow cytometry immunoblot assay were used assess apoptosis...

The transformation of primary rat cells into established cell lines by eimian virus 40 has been monitared using the different restrictive assays of colony formation in sparse culture, dense colony or focus formation on a confluent cell sheet, and colony formation in semisolid medium. Primary embryonic rat cell cultures are considerably less susceptible to infection and subsequent transformation...