نتایج جستجو برای: colony assay

تعداد نتایج: 273197  

Journal: :Blood 1985
T W LeBien D E Stepan R M Bartholomew R C Stong J M Anderson

We have previously used a chromium-release assay to demonstrate that the cocktail of monoclonal antibodies BA-1, BA-2, BA-3, and complement can effectively lyse human leukemic cells in the presence of excess bone marrow. Using a leukemic cell colony assay, we have reinvestigated the variables influencing lysis of human leukemic cells (KM-3, HPB-NULL, NALM-6) in bone marrow using BA-1, BA-2, BA-...

2006
Toshihiro Shibata Osamu Nakayama Masakuni Okuhara Yasuhisa Tsurumi Hiroshi Terano Masanobu Kohsaka

The colony forming units in culture (cfu-c) in bone marrowcells, which were suppressed by immunosuppressive factor obtained from the serum of sarcoma 180 tumor bearing mouse, was restored to normal level by the addition of FR-900490in vitro. Furthermore, in mitomycin C (MMC)-treated mice the subsequent administration of FR-900490caused a significant increase of cfu-c in bone marrow cells depres...

2005
By S. J. Knox F. D. Wilson B. R. Greenberg M. Shifrine L. S. Rosenblatt J. D. Reeves

In vitro radiation survival of peripheral blood T lymphocytes was studied in 1 5 clinically normal adults and 4 patients with Fanconi’s anemia. Tritiated thymidine incorporation in a whole blood lymphocyte stimulation test (1ST) and a newly developed whole blood T-lymphocyte colony assay were used to measure lymphocyte blastogenesis and colony formation in response to phytohemagglutinin (PHA) o...

Journal: :journal of paramedical sciences 0
samideh khoei cellular and molecular research centre, medical physics department, school of medicine, iran university of medical sciences, tehran maryam azarian department of biology, north tehran branch, islamic azad university, tehran maryam rafipour department of virology, pasteur institute of iran, tehran

the purpose of this study is to investigate the effects of quercetin as an inhibitor of heat shock proteins and hyperthermia on the induced dna damages and colony formation ability of du145 tumor spheroid culture. du145 cells were cultured as spheroids. on day11, spheroids with mean diameter 100 μm were treated with different concentration of quercetin for 24 hours and then exposed to hyperther...

Journal: :Tropical Journal of Pharmaceutical Research 2022

Purpose: To investigate the function and interaction of heat-shock cognate protein 70 (Hsc70) potassium voltage-gated channel subfamily A member 5 (KCNA5) in polycystic ovary syndrome (PCOS).Methods: Quantitative reverse transcription polymerase chain reaction (qRT-PCR) western blot were used to measure Hsc70 KCNA5 expression PCOS. The effect on granulosa cell apoptosis was determined by flow c...

Journal: :General Physiology and Biophysics 2021

Neuroblastoma (NB) is an extracranial solid malignancy in childhood. More and more studies have demonstrated that circRNAs are essential regulators of various tumors. This study conducted to explore the role mechanism circular RNA CUT-like homeobox 1 (circCUX1) NB. The levels circCUX1, miR-338-3p plant homeodomain finger protein 20 (PHF20) were detected by qRT-PCR or Western blot. Cell prolifer...

Journal: :iranian journal of microbiology 0
ehsan khalili department of clinical biochemistry, school of medicine, tehran university of medical sciences, tehran, iran. vahid hosseini department of clinical biochemistry, school of medicine, tehran university of medical sciences, tehran, iran. roya solhi department of clinical biochemistry, school of medicine, tehran university of medical sciences, tehran, iran. mahdi aminian department of clinical biochemistry, school of medicine, tehran university of medical sciences, tehran, iran and recombinant vaccine research center, tehran university of medical sciences, tehran, iran.

background and objectives: numerous procedures in biology and medicine require the counting of cells. direct enumer- ation of colony forming units (cfus) is time-consuming and dreary accurate cell counting on plates with high numbers of cfus is error prone. in this study we report a new indirect cell counting method that was developed based on the use of redsafe fluorometric assay. the usefulne...

Journal: :Cancer research 1987
Y Asano T Shibuya S Okamura S Yamaga T Otsuka Y Niho

The effects of human recombinant granulocyte/macrophage colony-stimulating factor (GM-CSF) and human native purified granulocyte colony-stimulating factor (G-CSF) on the growth of clonogenic leukemic blast cells from eight Japanese patients with acute myeloblastic leukemia were studied, using an in vitro leukemic blast colony assay. The results showed that GM-CSF stimulated leukemic blast colon...

Journal: :Blood 1982
A W Burgess N A Nicola G R Johnson E C Nice

A microculture assay for murine granulocyte-macrophage colony-stimulating factor (GM-CSF) has been developed using fetal liver GM colony-forming cells (CFC) isolated by fluorescence-activated cell sorting. These GM-CFC are free of mature hemopoietic cells, such as granulocytes and macrophages, which may interfere with direct assays for GM-CSF. The assay procedure allows the quantitation of GM-C...

Babak Qasemi-Panahi Mahmood Ahmadi Hamedani Mansoureh Movahedin Mohammad Hassan yousefi Parviz Tajik, Reza Narenji Sani Shiva Shafiei

The complex process of spermatogenesis is regulated by various factors. Studies on spermatogonial stem cells (SCCs) have provided very important tool to improve herd genetic and different field. 0.2 to 0.3 percent of total cells of seminiferous tubules is consist of spermatogonial stem cells. To investigate and biomanipulation of these cells, proliferation and viability rate of cells should be ...

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