OBJECTIVE To compare an alternative method to the most probable number (MPN) test for the detection of total coliform present in manually expressed human milk. METHODS 343 samples of manually expressed human milk from flasks donated to the Human Milk Bank of Instituto Fernandes Figueira--IFF were sent to the Laboratory of Food Control of IFF. The samples were used for comparing both methods, ...

Hoyer, M.V., J.L. Donze, E.J. Schulz, D.J. Willis and D.E. Canfield, Jr. 2006. Total coliform and Escherichia coli counts in 99 Florida lakes with relations to some common limnological factors. Lake and Reserv. Manage. 22(2):141-150. The degree of bacterial contamination of 99 Florida lakes was studied using total coliform and Escherichia coli (E. coli) counts as indicators. Over 75% of the 405...

BACKGROUND Indiscriminate disposal of untreated wastes which are often heavily laden with sewage microorganisms some of which are pathogenic to humans into aquatic environments near cities could serve as potential dangers to human health. OBJECTIVE A prospective study was undertaken to investigate the scope of potential bacterial pathogens and to assess the extent of pollution of the Douala l...

Genetically feathered and featherless sibling broilers selected for matched BW were killed, scalded, and defeathered to determine the consequences of feathers and empty feather follicles on the recovery of bacteria from carcass breast skin. In trial 1, the vents of all carcasses were plugged and sutured before scalding to prevent the expulsion of cloacal contents during picking. In trial 2, hal...

The influence of volatile fatty acids on the ecology of the bacterial flora of the mouse intestinal tract has been studied in three situations where large fluctuations in the composition of the microflora have been observed. Young mice were shown to ingest solid food particles when 11 days old; this correlated with the appearance of strictly anaerobic fusiform bacilli in the intestinal lumen an...

Low-strength saline wastewater may be generated by tourist facilities, industries and communities located in coastal areas. Sea salts, mostly chlorides, when present in wastewaters at high concentrations, can cause inhibition on biological treatment processes. In this study, a laboratory down-flow anaerobic fixed bed reactor (DFAFBR) was used for treating saline wastewater. This wastewater was ...

Non-absorbable antibiotics, neomycin sulfate or polymyxin B, prevent death from an otherwise lethal dose of whole body radiation by suppressing the activity of the Gram-negative bacterial flora of the intestinal tract. The protective effect of such suppression has been evaluated over a range of radiation exposure from 325 to 675 r. Coliform-free animals uniformly survive exposure to 550 r, a do...

Three different approaches for establishing guidelines for the microbiological quality of treated wastewater that is reused for agriculture are reviewed. These approaches have different objectives as their outcomes: the absence of faecal indicator organisms in the wastewater, the absence of a measurable excess of cases of enteric disease in the exposed population and a model-generated estimated...