Phosphate-binding protein(s) was found in the inner mitochondrial membrane of calf heart by Sephadex G-200 and G-25 gel filtration. The binding activity was inhibited by N-ethylmaleimide and competed by a large amount of cold phosphate. The amount of phosphate bound to the fraction was 29 nmoles per mg of protein. Affinity chromatography with phosphate-bound Sepharose 4B confirmed the presence ...

Although several models have been proposed to account for how cytoskeleton polymerization drives protrusion in cell motility, the precise mechanism remains controversial. Here, we show that, in addition to force exerted directly against the membrane by growing filaments, the way elongating filaments pack also contributes to protrusion by generating an expansion of the cytoskeleton gel. Tomograp...

A double antibody radioimmunoassay (RIA) for rat apolipoprotein A-I is reported. The ApoA-I isolated from delipidated HDL by gel filtration yielded a single band on polyacrylamide gel electrophoresis in sodium dodecyl sulfate (SDS), and its amino acid composition resembled that reported by others. ApoA-I was iodinated by lactoperoxidase and the resulting 125I-apoA-I was purified by gel filtrati...

Pyrolysis of kitchen based vegetable waste (KVW) was studied in a designed packed bed reactor. The effect of process parameters like temperature, time and catalyst on bio-gas yield and its composition was studied. The total bio-gas yield was found to be maximum with non-catalysed operation (260ml/g) at 1073K (180min). Higher hydrogen (H(2)) yield with non-catalysed operation (32.68%) was observ...

Blood can form a gel when there are high concentrations of proteins, such as IgM and/or IgG antibodies, that precipitate at low temperatures (cryoglobulins) or agglutinate red blood cells (cold agglutinins) (1, 2 ). A bone marrow biopsy performed on this patient revealed Waldenström macroglobulinemia, which produces large amounts of IgM (3, 4). Warming this patient’s sample in a water bath, kee...

Peak parking experiments were conducted to study the chromatographic behavior in a RPLC system consisting of a column packed with superficially porous C(18)-particles and a mixture of methanol and water (70/30, v/v). The values of the surface diffusion coefficient and the retention equilibrium constant of a column packed with superficially porous C(18)-particles were comparable to those of col...

There is a vivid debate on the relative importance of local and regional factors in shaping microbial communities, and on whether microbial organisms show a biogeographic signature in their distribution. Taking a metacommunity approach, spatial factors can become important either through dispersal limitation (compare large spatial scales) or mass effects (in case of strongly connected systems)....

In this research, aerial parts of Euphorbia denticulata Lam were collected from Sanandaj city in Kurdistan province at the West of Iran. It was extracted by maceration using acetone as solvent. Isolation of compounds were carried out with repeated column chromatography using silica gel and normal preparative HPLC using YMC-Pack-Sil column and hexane: ethyl acetate as mobile phase. The structure...

Highlights Aromatic compounds generated 72.6% of the total odor activity values (OAVs) over time. Cold treatments had significantly lower OAVs than hot regardless age bedded pack. Abstract . Beef cattle producers are beginning to raise in confinement facilities such as slatted-floor barns, hoop and mono-slope facilities. Hoop typically use bedding packs part their manure management system, with...

Liquid 3 He at low temperatures is an ideal substance to study because of its natural purity. The superfluid state, which appears at temperatures below 3 mK, has many unusual and exciting properties. These have extensively been studied experimentally, and the results are in many cases well understood theoretically using quasiclassical approximation. In this work we study the case that liquid 3 ...