Most studies using rabbit or mouse antisera failed to detect CRI between human IgM directed to MAG. We show here that 9 of 10 such IgM express a public CRI as defined by a nonhuman primate antiserum. Shared idiotype is likely involved in (or close to) the combining site of those IgM since antiidiotypic serum inhibited the binding of IgM to MAG and reacted with IgM having different variable regi...

Using a panel of antibodies specific for H and L chain variable region subgroups, a panel of human monoclonal cold agglutinin (CA) and rheumatoid factor (RF) autoantibodies were analyzed. The vast majority of the two types of autoantibodies utilized VkIII L chains, many of which probably derive from the Humkv325 gene. However, while most RFs (77%) utilized VHI H chains, all the CAs used VHII su...

The amount of lysis effected by cold agglutinins is directly related to the ability of the antibody to initiate complement activation. This ability is modified by the concentration of antibody, its thermal amplitude (the highest temperature at which the antibody will react with the cell), the degree to which antibody fixation is modified by the presence of complement components (particularly C3...

42 human sera showing in vitro cytotoxic activity of restricted or broad HL-A specificities with test human lymphocytes were studied for the molecular and immunoglobulin class of cytotoxic antibody using sucrose gradient separations, DEAE-cellulose chromatography, and Sephadex G-200 gel filtration. Sera originated from patients with previous multiple pregnancies (19), multiply transfused patien...

Although cases of primary non-bacterial pneumonia of unknown but probable vinal origin have been reported from Europe and America only meagre references to this condition have been made in Indian medical literature. Shone and Passmore (1943) have referred to an outbreak of pneumonitis associated with autohaemagglutination in Indian army personnel. No such cases have yet been reported from the i...

Most of the plasma proteins have been identified in normal synovial fluid (Schur and Sandson, 1963; Ropes and Bauer, 1953) but they are usually present in lower concentrations than those found in the plasma (Ropes and Bauer, 1953; Pekin and Zvaifler, 1964; Sundblad, Jonsson, and Nettelbladt, 1961). The higher molecular weight proteins, such as IgM macroglobulins, a2-macroglobulin, and fibrinoge...

A potent anti-idiotype serum produced in a rabbit immunized with the isolated heavy chains of an IgM cold agglutinin "Col" was rendered specific by solid-state adsorptions. The anit-Col idiotype was shown to bind specifically to both isolated Col heavy (mu) and light (kappa) chains as well as to intact Col IgM by three methods: (i) reversal of anti-idiotype inhibition of Col cold agglutinin in ...

A human homogeneous IgM/K cold agglutinin (CA) Sa is described, whose corresponding antigen on erythrocytes (RBC) was abolished by neuraminidase. This indicated that the antigen was related to N-acetylneuraminic acid, similar to Pr and Gd antigens. In contrast, this antigen was only partially destroyed by proteases, whereas Pr antigens are completely destroyed and Gd antigens are not influenced...

Recently, a new technique for myocardial protection that does not rely on hypothermia has been reported. In this method, the heart is continuously perfused with normothermic hyperkalemic blood cardioplegia during the cross-clamp period. Cardiac arrest is achieved and maintained using high levels of potassium. Hypothermia is not part of this technique; thus, the danger of hypothermia can be avoi...

Methods We analyzed medical records of 78 children, admitted to Chungnam National University Hospital and diagnosed with lobar pneumonia by chest X-rays between March 2010 and December 2011. White blood cells, C-reactive protein (CRP), procalcitonin (PCT), specific antibodies to M. pneumoniae, and cold agglutinin (CA) were measured at admission. Children were divided into 2 groups: those with M...