BACKGROUND Cockroaches are of vital importance medically and hygienically as they can disperse human pathogenic agents and are especially responsible for food contamination and spreading of food borne pathogens. In this study, part of mtDNA-COI gene of five common pest cockroaches was tested for diagnostic and phylogenetic purposes. METHODS We have described barcode region of mtDNA-COI gene o...

Whereas most traditional classifications identify Ischnocera as a major suborder of lice in the order Phthiraptera, a recent molecular study based on one gene did not recover monophyly of Ischnocera. In this study we test the monophyly of Ischnocera using sequences of portions of three different genes: two nuclear (EF1 alpha and 18S) and one mitochondrial (COI). Analysis of EF1 alpha and COI se...

BACKGROUND Dirofilaria immitis, a filarial nematode, is the most important parasite-affecting dogs, causing cardiopulmonary dirofilariasis. Current diagnostic tools for detecting D. immitis include morphological assays, antigen detection, and X-ray. Herein, we developed a method for the molecular detection of D. immitis in blood using polymerase chain reaction (PCR). METHODS The study was con...

Abstract The fall armyworm (FAW), an invasive pest of maize, is emerging threat in Southern Asia after America and Europe. Recently, this notorious has also been found different areas Pakistan. To assess its presence Pakistan, a survey was carried out the provinces Punjab, Sindh, Khyber Pakhtunkhwa during May–October 2019. We observed highest incidence FAW Sindh with maximum impact districts Ta...

Simultaneous studies of both nuclear and mitochondrial markers were undertaken in two widespread Indo-West Pacific (IWP) marine invertebrates to compare and contrast the ability of these markers to resolve genetic structure. In particular, we were interested in the resolution of a genetic break between the Indian and Pacific Oceans due to historical isolation. Sequence variation from the nuclea...

This study provides evidence on integrating the morphological, field, and laboratory data, and application of the cytochrome oxidase subunit I (COI) barcoding gene to the three asexual or sexual Lysiphlebus spp., i.e., Lysiphlebus cardui (Marshall), Lysiphlebus confusus Tremblay and Eady and Lysiphlebus fabarum (Marshall) (Hymenoptera: Braconidae: Aphidiinae). New aphid- invasive plant associat...

Codon usage bias (CUB) refers to an unequal occurrence of specific synonymous codons in a genome with variations within and among species. Findings suggest that CUB is significant interpreting evolutionary trends species and/or genes. However, information remained unclear for organisms the family Potamididae. This study examined compositional features codon patterns coI gene potamidids identify...

The aim of this study was to evaluate the utility of COI, of the mitochondrial DNA, for the taxonomic determination of soft-bottom benthic polychaetes in the Chiloé Inner Sea as a particularly important zone for polychaete fauna of the southeast Pacific. The results show high genetic differentiation between morphological species of polychaetes (posterior probabilities upper 0.9), and Onuphis ps...

This study reports on the molecular identification of fish species from processed products which had a priori been classified as belonging to 5 important species in Iran for human consumption. DNA barcoding using direct sequencing of an approximately 650bp of mitochondrial Cytochrome oxidase subunit I (COI) gene revealed incorrect labeling of Narrow-barred Spanish mackerel samples. High occurre...

The upper reaches of the Yangtze River (upper YR) are a biological zone with extremely rich fish diversity, especially endemic fish. However, long-term human interference, such as environmental pollution and cascade hydropower construction, has significantly changed habitat many is threatening number genetic diversity populations. Jinshaia sinensis typical small but rare that found in YR, its s...