Three commercially available rapid slide agglutination tests for the identification of Staphylococcus aureus were evaluated with 354 recent clinical isolates (165 strains of S. aureus). The test results of two latex agglutination products, SeroSTAT Staph (Scott Laboratories, Inc.) and Staphylatex (American Micro Scan), and one hemagglutination product, Staphyloslide (BBL Microbiology Systems), ...

The incidence of nosocomial infections with methicillin-resistant Staphylococcus aureus is of great concern in Japan and the developed world as a whole. Simple typing techniques like coagulase and phage typing are quick and useful for monitoring and evaluating these organisms. In view of this, the current status of antimicrobial susceptibility in Staphylococcus aureus (S. aureus) isolates in Ok...

PURPOSE To determine whether a 3-day application of ofloxacin results in the selection of fluoroquinolone-resistant conjunctival coagulase-negative Staphylococcus. DESIGN Prospective randomized trial. METHODS Patients scheduled for ocular surgery were randomized to a control (89 eyes) or study group (70 eyes). The study group received topical ofloxacin (0.3%) four times a day for 3 days. Co...

Numerous investigations have attempted to define those factors responsible for the virulence of Staphylococcus aureus.'" Almost all of the enzymes and exotoxins produced by staphylococci have at some time been purported to be responsible for their pathogenicity but none has been clearly demonstrated of paramount importance.'" The relation of coagulase and alpha hemolysin to the pathogenesis of ...

The role of coagulase in staplhylococcal infections is tincertain. It has been clemonstrated(1) that coagulase-negative hemolytic staphylococci wllen treated witlh coagulase and injected intracerebrally into mice exlhibit a higlher degree of virulence than the same organisms without treatment with coagulase. Other workers(2,3) have also found an association of coagtulase with virulence. However...

1 2 The cell surface-associated extracellular adherence protein (Eap) mediates adherence of 3 Staphylococcus aureus to host extracellular matrix components, and inhibits inflammation, 4 wound healing and angiogenesis. A well-characterized collection of S. aureus and non5 S. aureus staphylococcal isolates (n = 813) was tested for the presence of the Eap-encoding 6 gene (eap) by PCR to investigat...

We evaluated the performance of the Vitek MS for identification of Gram-positive cocci routinely isolated in clinical microbiology laboratories. With a total of 424 well-characterized isolates, the results of the Vitek MS were compared to those of conventional methods and 16S rRNA gene sequencing. The Vitek MS correctly identified 97.9 % of the isolates tested to species level. The Vitek MS cor...