" Coagulase Plates" When human blood bank plasma is mixed with nutrient agar in a concentration of about 12 to 15O% v v and poured into Petri dishes at a temperature of 45° C. the mixture sets as a clear gel. Staphylococci inoculated on to this medium develop opaque haloes around the colonies in 12 to 48 hours if they produce coagulase (Lack and Wailling, 1954). The advantages of the plate meth...

AIM To develop and evaluate a rapid enzyme linked immunosorbent assay (ELISA) for the diagnosis of intravascular catheter related sepsis caused by coagulase negative staphylococci. METHODS Forty patients with a clinical and microbiological diagnosis of intravascular catheter related sepsis and positive blood cultures, caused by coagulase negative staphylococci, and 40 control patients requiri...

BACKGROUND AND OBJECTIVES Coagulase-negative Staphylococcus species is the most common cause of peritoneal dialysis-related peritonitis; however, the optimal treatment strategy of coagulase-negative Staphylococcus species peritonitis remains controversial. DESIGN, SETTING, PARTICIPANTS, & MEASUREMENTS All of the coagulase-negative Staphylococcus species peritonitis in a dialysis unit from 199...

This strain appeared to consist of a mixture of two variants: 4 of 20 subcultures from single colonies were coagulase-negative; the remaining 16 were coagulase-positive. The two variants differed also in respect to toxin production. The hemolytic zones produced on 5% rabbit blood agar by the coagulasenegative variant were smaller than the zones produced by the coagulase-positive one. This prope...

A selective, differential plating medium was developed for the isolation and identification of coagulase-positive and mannitol-fermenting staphylococci. Coagulase produced by growing Staphylococcus aureus caused an opaque zone of fibrin to form around each colony. Several strains of S. aureus produced a visible coagulase reaction by 8 hr, and all strains gave a positive reaction before 12 hr. M...

Staphylococcus aureus (including Methicillin-resistant S. aureus) and coagulase negative Staphylococcus spp (including methicillin-resistant coagulase negative) permanently or transiently colonize humans and act as sources of complicated skin and soft tissue infections, bacteraemia and infective endocarditis. They also cause chronic infection of indwelling medical devices. Treatment of these in...

Slime-producing coagulase-negative staphylococci are pathogens in vascular surgery by virtue of their ability to adhere to and persist on prosthetic graft material. Inguinal and abdominal skin sites were cultured in 41 patients upon hospitalization, and slime production and antimicrobial susceptibility were assessed in all recovered staphylococcal isolates. Twenty-one patients eventually underw...

negative strains according to the slide clumping reaction, were the test organisms. In the clumping factor-positive series, there were 13 coagoulase-positive (including the Smith strain) and three coagulase-negative organisms. The clumping factor-negative group was composed of eight coagulase-positive and six coagulase-negative staphylococci. Of the coagulase-positive, clumping factor-negative ...

BACKGROUND Bloodstream infections in neonates and infants are life-threatening emergencies. Identification of the common bacteria causing such infections and their susceptibility patterns will provide necessary information for timely intervention. This study is aimed at determining the susceptibilities of bacterial etiological agents to commonly-used antimicrobial agents for empirical treatment...

Dye-diffusion test for semiquantitative staphylocoagulase assay. Ever since 1903, when Leob reported the ability of staphylococci to produce coagulase, an enzyme capable of clotting plasma, numerous workers have demonstrated the practical applicability of this phenomenon as an indicator of potential pathogenicity of these organisms. Two general procedures for coagulase determination are the tub...