In eukaryotes, although theMcm2-7 complex is a key component of the replicative DNA helicase, its association with Cdc45 and GINS (the CMG complex) is required for the activation of the DNA helicase. Here, we show that the CMG complex is localized to chromatin in human cells and describe the biochemical properties of the human CMG complex purified from baculovirus-infected Sf9 cells. The isolat...

Clinical metagenomics (CMg), referred to as the application of next-generation sequencing (NGS) to clinical samples, is a promising tool for the diagnosis of hospital-acquired pneumonia (HAP). Indeed, CMg allows identifying pathogens and antibiotic resistance genes (ARGs), thereby providing the information required for the optimization of the antibiotic regimen. Hence, provided that CMg would b...

In eukaryotic DNA replication initiation, hexameric MCM (mini-chromosome maintenance) unwinds the template double-stranded DNA to form the replication fork. MCM is activated by two proteins, Cdc45 and GINS, which constitute the 'CMG' unwindosome complex together with the MCM core. The archaeal DNA replication system is quite similar to that of eukaryotes, but only limited knowledge about the DN...

DNA replication licensing is now understood to be the pathway that leads to the assembly of double hexamers of minichromosome maintenance (Mcm2-7) at origin sites. Cell division control protein 45 (Cdc45) and GINS proteins activate the latent Mcm2-7 helicase by inducing allosteric changes through binding, forming a Cdc45/Mcm2-7/GINS (CMG) complex that is competent to unwind duplex DNA. The CMG ...

Overactive bladder (OAB) can be caused by a variety of conditions. We believe that cystometrography (CMG) is an essential part of the diagnostic evaluation, both in defining underlying pathophysiology and directing treatment. Essential to the diagnosis of OAB syndrome is some combination of urinary frequency, urgency, urge incontinence, and pain. CMG can be thought of simply as a provocative te...

Control moment gyros (CMGs) are spacecraft attitude control actuators which act as torque amplifiers. They are thus suitable for attitude hold and reorientation of large spacecraft or for slew maneuvering. They provide the necessary torques via gimballing a spinning flywheel. A major problem encountered with the use of CMGs in practice is the possibility of singularities for certain combination...

Satellite-derived snow maps from NASA’s Earth Observing System Moderate Resolution Imaging Spectroradiometer (MODIS) have been produced since February of 2000. The global maps are available daily at 500-m resolution, and at a climate-modeling grid (CMG) resolution of 1/20o (~5.6 km). We compared the 8-day composite CMG MODIS-derived global maps from November 1, 2001, through March 21, 2002, and...

Two-wheeled robots are popular in transportation applications because of their high maneuverability. In this research, the oscillation attenuation performance control moment gyroscope (CMG) for two-wheeled robot was studied. The stored kinetic energy a CMG can offer weight and volume saving compared to conventional vibration absorbers. This is also more reactionless than other absorbers by tran...

Control of systems with operating condition-dependent dynamics, including control moment gyroscopes (CMGs), often requires controllers to achieve high performance. The aim this brief is develop a frequency response data-driven linear parameter-varying (LPV) design approach for single-input single-output (SISO) systems, which allows improved performance CMG. A stability theory using closed-loop ...