Muscular tissue regeneration may be enhanced in vitro by means of mechanical stimulation, inducing cellular alignment and the growth functional fibers. In this work, a novel bioreactor is designed for radial stimulation porcine-derived diaphragmatic scaffolds aiming at development clinically relevant patches. A Finite Element (FE) model membrane developed, considering two different methods grip...

The purpose of this study is to compare the performance of laparoscopic partial nephrectomy (LPN) with and without clamping of the renal artery and to evaluate the impact of clamping on postoperative renal function. A total of 20 patients underwent LPN, 13 without and 7 with clamping of the renal artery. The 2 groups were compared with respect to complications, blood loss, operative time, mean ...

Electrophysiological measurements, in particular, patch clamping, have long been regarded as the "gold standard" for assaying ion channels. Despite its high information content, the technique suffers from laborious, manual processing by highly skilled workers and extremely low throughput. Recently, a number of researchers have started to automate patch clamping by either automating conventional...

BACKGROUND The purpose of vascular clamping during the course of liver resection is to reduce bleeding and subsequent complications. AIM To show both step-by-step surgical techniques for vascular exclusion of the liver and their indications. METHODS It is described the following techniques: clamping of the hepatic pedicle, "Pringle" maneuver; intermittent clamping of the hepatic pedicle; in...

For at least over 200 years, multiple controversies have arisen around the timing of umbilical cord clamping. In the past decades, early cord clamping (within the first 15 seconds) had markedly prevailed. Only in the 21st century, randomized controlled trials have demonstrated the benefits of delayed cord clamping (at 2-3 minutes). Delayed cord clamping has been practiced in obstetrics based on...

BACKGROUND This retrospective study was designed to investigate the efficacy and safety of intermittent portal triad clamping (PTC) with low central venous pressure (CVP) in liver resections. METHODS Between January 2007 and August 2013, 115 patients underwent liver resection with intermittent PTC. The patients' data were retrospectively analyzed. RESULTS There were 58 males and 57 females ...

PURPOSE We evaluated the utility of a thulium laser and a novel implementation of its use for laparoscopic partial nephrectomy to achieve precise, smokeless, and hemostatic dissection without hilar clamping and with minimal charring in a porcine model. MATERIALS AND METHODS Laparoscopic transperitoneal lower-pole partial nephrectomy was performed in five Yorkshire farm pigs without clamping o...

Recently, the American College of Obstetricians & Gynecologists (ACOG) recommended a 30–60 second delay in umbilical cord clamping for all preterm deliveries [1]. Delayed cord clamping in preterm infants has been associated with a greater than 50% reduction in intraventricular hemorrhage and less need for blood transfusions as demonstrated in a recent Cochrane meta-analysis of 10 randomized con...

RATIONALE Remote ischemic preconditioning (RIPC) has been suggested to induce cardioprotection during cardiac surgery. Maintaining proper atrial function is imperative in preventing arrhythmia and thrombus formation. Mitochondria have been proposed as key targets in conveying RIPC mechanisms and effects. MicroRNA (miR) is emerging as an important regulator of mitochondrial function, arrhythmia,...