Using herbal drugs is the common way for treatment of diseases in traditional and alternative medicine. These drugs have important role and strong cultural background among people in treatment of hyper-billirubinemia. It is necessary to evaluate the effect of herbal drugs by scientific methods. The goal of this study is evaluation the effect of common herbal drugs used in management of neonatal...

Chicory (Cichorium intybus L.), which is known to have a variety of terpene-hydroxylating activities, was screened for a P450 mono-oxygenase to convert (+)-valencene to (+)-nootkatone. A novel P450 cDNA was identified in a chicory root EST library. Co-expression of the enzyme with a valencene synthase in yeast, led to formation of trans-nootkatol, cis-nootkatol and (+)-nootkatone. The novel enz...

In young chicory plantlets [Cichorium intybus L. Witloof cv. Flash), nitrate assimilation takes place mainly in the roots. Nitrate reductase activity (NRA) was measured in roots deprived of shoot control by excision and transferred into a sucrose-containing medium. Such a treatment resulted in a drop of about 60% of NRA within 3 h. The level of NR protein decreased after 12 h and the level of N...

A "novel" protocol is presented for easy and reliable estimation of soluble hydroxycinnamate levels in Cichorium intybus L. leaf tissue in large-scale experiments. Samples were standardized by punching 6 discs per leaf, and hydroxycinnamates were extracted by submerging the discs in 80% ethanol with 5% acetic acid for at least 48 h in the darkness at 4°C. Residual dry mass of the discs was used...

Pectins are major components of the primary plant cell wall. They can be both methylesterified and acetylesterified and de-esterification occurs by specific esterases. Proteins extracted by NaCl treatment from root cell walls of two chicory varieties (Cichorium intybus L. cv. Nausica and Arancha) sampled in an experimental field every 2 weeks between July 2002 and January 2003 were analysed by ...