Two hundred and forty Hubbard Classic broiler chicks were used in a 35 day trial at Chittagong Veterinary and Animal Science University farm, Bangladesh to study the effects of supplemental choline chloride on performance parameters, carcass characteristics and their association in commercial broiler. All birds had free access to ad libitum feeding. Birds were fed four types of diet i.e. diet w...

Mutagenesis studies on glucose oxidases (GOxs) were conducted to construct GOxs with reduced oxidase activity and increased dehydrogenase activity. We focused on two representative GOxs, of which crystal structures have already been reported—Penicillium amagasakiense GOx (PDB ID; 1gpe) and Aspergillus niger GOx (PDB ID; 1cf3). We constructed oxygen-interacting structural models for GOx...

Since, choline chloride caking causes serious operating difficulties and customer complaints, two experiments were conducted to optimize in vitro production of a novel encapsulated choline chloride (ECC) with minimum hygroscopic property and optimize delivery in gastrointestinal tract (GIT). The in vivo verification test of ECC was used to compare it with the commercial choline chloride (CC) in...

Being one of the most commonly used electrochemical mediators for analytical applications, Prussian Blue has found a wide use in the biosensor field during the last years. Its particular characteristic of catalysing hydrogen peroxide reduction has been applied in the construction of a large number of oxidase enzyme-based biosensors for clinical, environmental and food analysis. By modifying an ...

Pseudomonas aeruginosa can acquire and metabolize a variety of molecules including choline, an abundant host-derived molecule. In P. aeruginosa, choline is oxidized to glycine betaine which can be used as an osmoprotectant, a sole source of carbon and nitrogen, and as an inducer of the virulence factor, hemolytic phospholipase C (PlcH) via the transcriptional regulator GbdR. The primary objecti...

The influence of folic acid upon various enzyme systems both in vivo and in vitro has recently been investigated by workers in several laboratories. In this laboratory it has been demonstrated that folic acid depresses xanthine oxidase activity when added to the enzyme systems in vitro (1) or when fed to chicks (2). It has also been shown that n-amino acid oxidase activity is decreased by dieta...