نتایج جستجو برای: chlorophyll chloroform fumigation extraction cfe
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BACKGROUND Both shorter and longer telomeres in peripheral blood leukocyte (PBL) DNA have been associated with cancer risk. However, associations remain inconsistent across studies of the same cancer type. This study compares DNA preparation methods to determine telomere length from patients with colorectal cancer. METHODS We examined PBL relative telomere length (RTL) measured by quantitativ...
Sample preparation and DNA extraction protocols for DNA amplification by PCR, which can be applied in human fecal samples for taeniasis diagnosis, are described. DNA extracted from fecal specimens with phenol/chloroform/isoamilic alcohol and DNAzol reagent had to be first purified to generate fragments of 170 pb and 600 pb by HDP2-PCR. This purification step was not necessary with the use of QI...
obtained without a phenol/chloroform extraction; however, fewer errors, greater signal strength and longer reads are obtained from the inclusion of a phenol/chloroform extraction before the butanol precipitation (Figure 1, A and C). In conclusion, this paper presents a simple dye terminator sequencing product purification protocol. This protocol is standardly used in our laboratory and provides...
When freeze-dried brain was extracted at -4-0degrees C with dry chloroform/methanol (2:1 v/v), four of the five enzymes examined were recovered in the diethyl ether-washed residue without inactivation. By contrast, extraction with chloroform/methanol (2:1 v/v) in the presence of water destroyed activities of all the enzymes examined. The amounts of major lipids extracted were similar whether ex...
In this paper, a novel method for the determination of chloroform in drinking water has been described. It is based on liquid-phase microextraction (LPME) and gas chromatography-mass spectrometry (GC-MS). Extraction conditions such as solvent selection, organic solvent dropsize, stirring rate, content of NaCl and extraction time were found to have significant influence on extraction efficiency....
I. Methods. S1. Extraction of the non-hydrolyzed TG and reaction products by chloroform. The used procedure is taken from ref. (26). Briefly, after stopping the lipolysis reaction with Orlistat granules, the reaction mixture was allowed to cool down to room temperature and its pH was decreased to pH = 2 by adding HCl (to decrease the solubility of the reaction products in the aqueous phase). Ne...
Context Soxhlet extraction is commonly used for determining fat content of meat, because it the basis AOAC Final Action method this analysis. Petroleum ether as an solvent but other solvents, such chloroform, can be used. Chloroform has been stated to a safer than petroleum and extensively meat research in Australia; yet, there no report that validates its use purpose.Aim To compare chloroform ...
A lipid fraction was obtained by extraction with chloroform-methanol (1:1 .v/v) of "the S-precipitate"prepared from whole human saliva by one precipitation.This fraction produced leukocytosis, but not hypocalcemia , in rabbits upon intravenous injection. Saliva-parotin-A purified from the S-precipitate possessed both hypocalcemic and leukocytosis-promoting activities, which remained unaffected ...
The complex formation and a liquid-liquid extraction in the cobalt(II) 4-(2thiazolylazo)resorcinol (TAR) 2,3,5-triphenyl-2H-tetrazolium chloride (TTC) water chloroform system was studied. The optimum extractionspectrophotometric conditions were found. The molar ratio of the reacting species was determined to be 1:2:1 (Co:TAR:TTC). The following equilibrium processes were quantitatively examined...
The influence of hydrogen fluoride fumigation on water economy was studied using soybean (Glycine max [L] Merr.). Fumigation caused partial stomatal closure in 1 hour and practically complete closure within 4 hours. The transpiration rate was greatly reduced by fumigation, while the leaf temperature was increased. Water potential increased after 1 day but fell drastically when necrosis occurred...
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