Mitosis is a fundamental process in the development of all organisms. The mitotic spindle guides the cell through mitosis as it mediates the segregation of chromosomes, the orientation of the cleavage furrow, and the progression of cell division. Birth defects and tissue-specific cancers often result from abnormalities in mitotic events. Here, we report a proteomic study of the mitotic spindle ...

Four water-soluble Fusarium metabolites (fumonisin B1, fusaric acid, butenolide and moniliformin), water-insoluble pigment (8-O-methylbostrycoidin), and an Alternaria metabolite (AAL-toxin) were tested for relative cytotoxicity to five established mammalian cell lines. Butenolide was the most cytotoxic to all five cell lines. LC50s were; 1 microgram/ml to rat hepatoma (RH) (tumors derived from ...

The Chinese hamster ovary cell line xrs-1 is hypersensitive to gamma-radiation. This sensitivity has been attributed to an inability of this cell line to efficiently repair gamma-ray induced double-strand breaks (DSBs). We have recently reported that xrs-1 is also sensitive to topoisomerase II inhibitors that stabilize the cleavable complex. In this study, we have investigated the basis of this...

The ability of glycopeptides, isolated from bovine cerebral cortex, to alter cell division was studied by cell-cycle analyses. The results showed that glycopeptides arrested baby hamster kidney (BHK)-21 cells and Chinese hamster ovary (CHO) cells in the G2 phase of the cell cycle. Upon removal of the growth inhibition from arrested BHK-21 cells, the mitotic index in colchicine-treated cultures ...

Introduction Chinese Hamster Ovary (CHO) cells are widely used for the large scale production of recombinant biopharmaceuticals. These cells have been extensively characterised and approved by regulatory authorities for production of biopharmaceuticals. During the last years more and more cell-line engineering strategies have been developed to enhance productivity and quality. CHO cell line eng...

Neuroblastoma cells had a much higher rate of glycolysis than the Chinese hamster ovary Kl cells, although the former cell type had a longer doubling time (24 hr) than did the latter (12 hr). DL-Glyceraldehyde inhibited the glycolysis in both cell types to the same extent, but it reduced the growth of neuroblastoma cells more markedly than that of Chinese hamster ovary Kl cells. These data indi...

Recently, we cloned and characterized a full-length cDNA of the hamster Muc1 gene, the expression of which appears to be associated with secretory cell differentiation (Park HR, Hyun SW, and Kim KC. Am J Respir Cell Mol Biol 15: 237-244, 1996). The role of Muc1 mucins in the airway, however, is unknown. In this study, we investigated whether cell surface mucins are adhesion sites for Pseudomona...

Abbreviations: CPRG, chlorophenol red-β-D-galactopyranoside; CFP, Cyan Fluorescent Protein; CHO, Chinese Hamster Ovary cells; FRET, Fluorescence Resonance Energy Transfer; GFP, Green Fluorescent Protein; HEX, hexachloro-carboxyfluorescein; HUVEC, Human Umbilical Vein Endothelial Cells; RHD, Rel Homology Domain; TET, tetrachloro-carboxyfluorescein; β-TrCP, beta-transducin repeat-containing prote...

In the present study, we have identified species-specific housekeeping genes (HKGs) for Chinese Hamster Ovary (CHO) cells using data from microarray gene expression profiling. HKGs suitable for quantitative RT-PCR normalization should display relatively stable expression levels across experimental conditions. We analyzed transcription profiles of several IgG-producing recombinant CHO cell lines...

ISSN 2048-9145 Recombinant proteins produced via Chinese hamster ovary (CHO) cell cultures constitute the majority of biotherapeutics on the market. The ability of these cultures to consistently perform human-like post-translational modifications, mainly glycosylation, is paramount to product quality. Although much research has focused on understanding enzymatic function and regulation in the g...