نتایج جستجو برای: chinese hamster ovary cell line

تعداد نتایج: 2121057  

Journal: :anatomical sciences journal 0
hamid karimi university of tabriz, faculty of veterinary medicine, mohsen abbasi fatemeh balazadehkocheh belal hasanzadeh

introduction: genital system is important herd in animal's creation. it is necessary for animal reproduction. mammal has two sex in genital system such as male and female in separated animals. animal reproduction destroyed by disorder of genital system. in this case, animals cannot produce an animal similar to them. finally it cause overthrow a species of animals. methods: histomorphologic...

Journal: :The Journal of biological chemistry 1986
P P Saunders M T Tan C D Spindler R K Robins W Plunkett

3-Deazaguanosine containing a 14C label in the ribose moiety was prepared using [U-14C]inosine as the [14C] ribose donor and commercial purine-nucleoside phosphorylase (EC 2.4.2.1) both to degrade the inosine, in the presence of phosphate, and to synthesize [14C-ribosyl]3-deazaguanosine in reduced phosphate and an excess of 3-deazaguanine. Purification was by high-pressure liquid chromatography...

Amiri Yekta A Bahrami Nejad E Fatemi N, Gourabi H Rahnama M, Sanati MH

Background: The demand for producing recombinant therapeutic proteins by mammalian cell lines, such as Chinese hamster ovary (CHO) cells, continues to grow. Significant achievements in process optimization including development of cell culture strategies for largescale and cost-effective production have been made. Fetal bovine serum (FBS) is an often essential growth supplement and yet most cos...

Journal: :Journal of virology 1988
D Spehner S Gillard R Drillien A Kirn

Cowpox virus, in contrast to vaccinia virus, can multiply in Chinese hamster ovary cells. To study the genetic basis for this difference in host range, recombinants between vaccinia and cowpox viruses were isolated and their DNA restriction patterns were examined. The ability to multiply in Chinese hamster ovary cells could be correlated with the conservation of cowpox virus sequences mapping a...

Journal: :Nature biotechnology 2013
Nathan E Lewis Xin Liu Yuxiang Li Harish Nagarajan George Yerganian Edward O'Brien Aarash Bordbar Anne M Roth Jeffrey Rosenbloom Chao Bian Min Xie Wenbin Chen Ning Li Deniz Baycin-Hizal Haythem Latif Jochen Forster Michael J Betenbaugh Iman Famili Xun Xu Jun Wang Bernhard O Palsson

Chinese hamster ovary (CHO) cells, first isolated in 1957, are the preferred production host for many therapeutic proteins. Although genetic heterogeneity among CHO cell lines has been well documented, a systematic, nucleotide-resolution characterization of their genotypic differences has been stymied by the lack of a unifying genomic resource for CHO cells. Here we report a 2.4-Gb draft genome...

Journal: :Cancer research 1990
H F Bligh A Bartoszek C N Robson I D Hickson C B Kasper J D Beggs C R Wolf

Mitomycin C is an alkylating agent used in cancer chemotherapy that shows some specificity towards hypoxic cells. The therapeutic effects of this compound are thought to result from its metabolic activation by enzymes such as NADPH:cytochrome P-450 reductase. In a previous report we described a Chinese hamster ovary cell line resistant to mitomycin C, which had a decreased NADPH:cytochrome P-45...

Journal: :Cancer research 1992
K V Speeg C deLeon W L McGuire

Many of the cytotoxic substrates of the multidrug transporter are organic cations. Cimetidine, procainamide, and tetraethylammonium bromide were used in a Chinese hamster ovary model of multidrug resistance, to study handling of noncytotoxic cationic transport probes. Cimetidine and procainamide, but not tetraethylammonium, accumulated to a greater extent (5-fold) in the sensitive CHOAUXB1 (AB)...

Journal: :Indian journal of experimental biology 2009
Chengfu Yuan Youquan Bu Joyeeta Sengupta Junxia Yang Xiuning Huang Li Cheng Qin Zhang Faping Yi Geli Liu Fangzhou Song

The whole length of MCHR2 gene cDNA fragment was amplified by PCR using human fetal brain cDNA library as template. The pcDNA3.1 (+)/MCHR2 eukaryotic expression vector was constructed successfully. The recombinant pcDNA3.1 (+)/MCHR2 plasmid was transfected into Chinese hamster ovary (CHO) cell by lipofectamine 2000, after G418 selection and then the CHO cell line expressing MCHR2 gene was estab...

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