BACKGROUND The anti-inflammatory effects of a homeopathic remedy, Traumeel S, have been observed in experimental and clinical studies; however, its antioxidant properties have not been elucidated. The aim of the present study was to evaluate the antioxidant effects of Traumeel S on peripheral blood neutrophils in patients with periodontitis. MATERIAL/METHODS The study was performed using veno...

On-line automation of two different liquid chromatographic procedures, a pre-column derivatization system and a pre- and post-column system, in order to generate chemiluminescence is reported. Dansyl chloride (Dns-Cl) was used as a pre-column reagent to form fluorophores and bis(2,4,6-trichlorophenyl) oxalate (TCPO) and hydrogen peroxide (H2O2) as a post-column reagent to generate chemiluminesc...

Stimulated neutrophils show ionic fluxes that may function as "transducers" between stimuli and effector functions. Using fluorescent probes, patterns of membrane-associated calcium (chlortetracycline, CTC) and membrane potential (3-3'-dipentyloxacarbocyanine, di-O-C5 (3)) in single living human neutrophils were observed with a fluorescence microscope fitted with an image intensifier and photom...

The antioxidant capacity of polyphenols (+)-catechin, (-)-epicatechin and myricetin, and of different types of red wines (Cabernet Sauvignon, Malbec and blended wine) was evaluated by three assays. (a) NADH oxidation by peroxynitrite (ONOO-): the ONOO- scavenging activity was higher for myricetin (IC50=35 microM) than for (+)-catechin (IC50=275 microM) and (-)-epicatechin (IC50=313 microM). (b)...

The electrochemistry and electrogenerated chemiluminescence (ECL) of a series of europium chelates, cryptates, and mixed-ligand chelate/cryptand complexes were studied. The complexes were of the following general forms:  EuL(4)(-), where L = β-diketonate, a bis-chelating ligand (such as dibenzoylmethide), added as salts (A)EuL(4), where A = tetrabutylammonium ion or piperidinium ion (pipH(+)); ...

Coumarin C343 (C(16)H(15)NO(4)), a dye used to enhance the weak chemiluminescence associated with lipid peroxidation, was conjugated to silica nanoparticles and entrapped in a sol-gel matrix to produce a nanosensor capable of enhancing low-level chemiluminescence by approximately 100%.

The stimulation of neutrophils by formylmethionyl-leucyl-phenylalanine results in a bimodal luminol-dependent chemiluminescence pattern. We observed, however, only a single peak chemiluminescence pattern in the response to the peptide when we used lucigenin as an amplifying substance. We suggest that lucigenin, the larger molecule, (510 daltons; luminol is 177 daltons) only exerts an extracellu...

Herein, we report a facile strategy for the synthesis of dual-functionalized graphene hybrids (A-H-GNs) based on interactions among graphene, N-(aminobutyl)-N-(ethylisoluminol) (ABEI) and hemin. The multilayers of a chemiluminescence reagent (ABEI) and a catalyst (hemin) were accumulated on the graphene surface, resulting in high chemiluminescence performance, good solubility and stability in a...

Hydroxylamine-O-sulfonic acid, a versatile reagent for organic synthesis, has been explored as an effective coreactant for luminol chemiluminescence. Co(2+) can dramatically enhance the chemiluminescence of this system. The sensitive detection of Co(2+), luminol and HOSA, including highly selective Co(2+) detection, is achieved.