To reveal the dynamic process of cellulose biodegradation and explore more potential cellulases, a microbiota (FPDM) with cellulose-degrading ability was cultivated, different stages filter paper degradation were compared. Ion chromatography comparative metagenomic sequencing revealed that diversity FPDM enhanced as hydrolysate length increased. Sporocytophaga Cohnella dynamically dominated syn...

BACKGROUND Enzymatic breakdown of lignocellulosic biomass is a known bottleneck for the production of high-value molecules and biofuels from renewable sources. Filamentous fungi are the predominant natural source of enzymes acting on lignocellulose. We describe the extraordinary cellulose-deconstructing capacity of the basidiomycete Laetisaria arvalis, a soil-inhabiting fungus. RESULTS The L....

Cellulases find increasing prominence in sustainable production of fuel and feedstock from lignocellulosic biomass. The purification and biochemical characterization of individual components of cellulase complex is important to understand the mechanism of their action for the solubilization of crystalline cellulose. In this study, an extra-cellular endoglucanase isolated from culture filtrate o...

Various saccharides and related substances were mixed with carthamin in aqueous solutions and their effects on red colour stability compared. At a low temperature range (5 °C), monoand disaccharides contributed to preservation of the carthamin red colouration. No monoand disaccharides could relieve carthamin from being bleached at a high temperature (30 °C). Among poly­ saccharides and their de...

Cel5A, an endoglucanase, was derived from the metagenomic library of vermicompost. The deduced amino acid sequence of Cel5A shows high sequence homology with family-5 glycoside hydrolases, which contain a single catalytic domain but no distinct cellulose-binding domain. Random mutagenesis and cellulose-binding module (CBM) fusion approaches were successfully applied to obtain properties require...

A cosmid gene library was constructed in Escherichia coli from genomic DNA isolated from the ruminal anaerobe Fibrobacter succinogenes AR1. Clones were screened on carboxymethyl cellulose, and 8 colonies that produced large clearing zones and 25 colonies that produced small clearing zones were identified. Southern blot hybridization revealed the existence of at least three separate genes encodi...

The filtration of air has attracted increasing attention during recent waves viral infection. This review considers published literature regarding the usage cellulose-based materials in devices, including face masks. Theoretical aspects are reviewed, leading to models that can be used predict relationship between structural features filter media and collection efficiency for different particle ...

microcerotermes diversus (silvestri) is a major pest of buildings and a wide variety of crops and trees in khouzestan province, because of consuming on materials containing cellulose. one new environmentally safe tactic for the control of subterranean termites is baiting world-wide. in laboratory bioassays, toxicity of imidacloprid bait at concentrations of 25, 50 and 100 ppm, was evaluated und...

Amino acid modifications of the Thermobifida fusca Cel9A-68 catalytic domain or carbohydrate binding module 3c (CBM3c) were combined to create enzymes with changed amino acids in both domains. Bacterial crystalline cellulose (BC) and swollen cellulose (SWC) assays of the expressed and purified enzymes showed that three combinations resulted in 150% and 200% increased activity, respectively, and...