this study was aimed to evaluate the efficacy of dipstick tests (leukocyte esterase and nitrite) in diagnosis of spontaneous bacterial peritonitis (sbp) in cirrhotic patients. forty six children with ascites hospitalized between 2009 and 2010 in children medical center were enrolled in this study. reagent strip assays for leukocyte esterase and nitrite were performed on ascetic fluid and the re...

background and objectives: contamination of cell lines and biological products is one of the major problems of cell culture techniques. rapid detection of mycoplasma contamination in cell culture is an important part of quality control standards in related laboratories. the aim of this study was to evaluate the efficacy of pcr in detection of myroplasma as contaminants in cell cultures and othe...

staphylococcus spp., is a common bacteria in cattle mastitis. detection of the low number of bacteria requires a sensitive method. we used multiplex polymerase chain reaction (m-pcr) assay to detect the genes encoding sea, seb, sec toxins, that are specific in s. aureus bacteria. the gene of 23s rrna that is conserved in all staphylococcus spp., also was used. due to the stiffness and low effec...