In observing Francisella tularensis interactions with nonphagocytic cell lines in vitro, we noted significant adherence, invasion, and intracellular growth of the bacteria within these cells. F. tularensis live vaccine strain invasion of nonprofessional phagocytic cells is inhibited by cytochalasin D and nocodazole, suggesting that both the actin and microtubule cytoskeletons are important for ...

background: activation of the ubiquitin-proteasome pathway in various malignancies, including colorectal cancer, is established. this pathway mediates the degradation of damaged proteins and regulates growth and stress response. the novel human gene, ube2q2, with a putative ubiquitin-conjugating enzyme activity, is reported to be overexpressed in some malignancies. we sought to investigate the ...

background: oct4b1, a novel variant of oct4, is expressed in cancer cell lines and tissues. based on our previous reports, oct4b1 appears to have a crucial role in regulating apoptosis as well as stress response [heat shock proteins (hsps)] pathways. the aim of the present study was to determine the effects of oct4b1 silencing on the expression of high molecular weight hsps in three different h...

objective(s):according to the prevalence of neoplastic diseases, there is a deep necessity for discovery of novel anticancer drugs in the field of medicinal chemistry. in the current study, a new series ofphenylthiazole derivatives(compounds 4a-4f) was synthesizedand theiranticancer activity was assessed in vitro. materials and methods:all synthesized derivatives were evaluated towards three hu...

fumonisins, a family of mycotoxins, are mainly toxic and carcinogenic. the present study was carried out to evaluate fumonisin b1 (fb1) effects on the production of inflammatory cytokines by gastric and colon cell lines. the study was performed on two cell lines under in vitro condition, including gastric epithelial cell line (ags) and human colon adenocarcinoma cell line (sw742). lipopolysacch...

cytokines influence cell cycle events, which in some but not all instances can be associated with melanoma progression. analysis of the g0/g 1 and s phase fractions of the cell cycle was used to assay the proliferative or inhibitory activity of cytokines against ten human melanoma cell lines, including pairs of cell lines derived from primary and metastatic tissue of individual patients. cytoki...

Enteroaggregative Escherichia coli (EAEC) is an important diarrheal pathogen and a cause of both acute and chronic diarrhea. It is a common cause of pediatric bacterial diarrhea in developing countries. Despite its discovery in 1987, the intestinal tropism of the pathogen remains unknown. Cell lines used to study EAEC adherence include the HEp-2, T-84, and Caco-2 lines, but they exhibit abnorma...

Assessment of the cellular effects of natural and/or synthetic products is one of the major goals in biological experiments. This assessment may be conducted at different levels; cellular viability, cellular biological activity, and cellular structure and morphology. Different assays are available for any of above purposes. It is becoming of a routine exercise to apply any of these assays, and ...

fourier transformed infrared spectroscopy (ftir) has extensively been used for biological applications. cisplatin is one the most useful antineoplastic chemotherapy drugs for a variety of different human cancers. one of the clinical problems in its application, which would consequently affect the therapeutic outcome of its application, is the occurrence of resistance to this agent. in this proj...