نتایج جستجو برای: cell immortalization

تعداد نتایج: 1684829  

2013
Gabriela Brumatti Marika Salmanidis Chung H Kok Rebecca A Bilardi Jarrod J Sandow Natasha Silke Kylie Mason Jolanda Visser Anissa M Jabbour Stefan P Glaser Toru Okamoto Philippe Bouillet Richard J D'Andrea Paul G Ekert

Deregulated expression of Hox genes such as HoxA9 is associated with development of myeloproliferative disorders and leukemia and indicates a poor prognosis. To investigate the molecular mechanisms by which HoxA9 promotes immortalization of hematopoietic cells, we generated growth factor dependent myeloid cells in which HoxA9 expression is regulated by administration of 4-hydroxy-tamoxifen. Mai...

2015
María José Pino-Barrio Elisa García-García Pablo Menéndez Alberto Martínez-Serrano

A better understanding of the molecular mechanisms governing stem cell self-renewal will foster the use of different types of stem cells in disease modeling and cell therapy strategies. Immortalization, understood as the capacity for indefinite expansion, is needed for the generation of any cell line. In the case of v-myc immortalized multipotent human Neural Stem Cells (hNSCs), we hypothesized...

Journal: :Blood 2010
Alison Y Swaims Francesca Khani Yingyu Zhang Arthur I Roberts Satish Devadas Yufang Shi Arnold B Rabson

Infection with the human T-cell leukemia virus-1 (HTLV-1) results in a variety of diseases including adult T-cell leukemia/lymphoma (ATL). Although the pathogenesis of these disorders is poorly understood, it involves complex interactions with the host immune system. Activation of infected T cells may play an important role in disease pathogenesis through induction of the oncogenic HTLV-1 Tax t...

Journal: :Frontiers in bioscience : a journal and virtual library 1998
J Russo X Yang Y F Hu B A Bove Y Huang I D Silva Q Tahin Y Wu N Higgy A Zekri I H Russo

Human breast cancer remains the most common malignancy in the American women. The ultimate cure of this disease relies on a better understanding of the mechanisms underlying the initiation and progression of this disease. The neoplastic transformation of HBEC in vitro represents a successful model for obtaining knowledge on the molecular and biological alterations that may contribute to the tum...

2014
Sofie Degerman Mattias Landfors Jan Konrad Siwicki John Revie Magnus Borssén Emma Evelönn Erik Forestier Krystyna H. Chrzanowska Patrik Rydén W. Nicol Keith Göran Roos

We have previously described gene expression changes during spontaneous immortalization of T-cells, thereby identifying cellular processes important for cell growth crisis escape and unlimited proliferation. Here, we analyze the same model to investigate the role of genome-wide methylation in the immortalization process at different time points pre-crisis and post-crisis using high-resolution a...

Journal: :The Journal of Cell Biology 2000
Elena Dellambra Osvaldo Golisano Sergio Bondanza Emanuela Siviero Pedro Lacal Marta Molinari Stefania D'Atri Michele De Luca

In human epidermal keratinocytes, replicative senescence, is determined by a progressive decline of clonogenic and dividing cells. Its timing is controlled by clonal evolution, that is, by the continuous transition from stem cells to transient amplifying cells. We now report that downregulation of 14-3-3sigma, which is specifically expressed in human stratified epithelia, prevents keratinocyte ...

Journal: :The Journal of general virology 2001
K Konishi S Maruo H Kato K Takada

To quantitatively evaluate the role of Epstein-Barr virus (EBV)-encoded latent membrane protein 2A (LMP2A) in immortalization of peripheral B-lymphocytes, we used the Akata cell system to generate an EBV recombinant in which the first exon of the LMP2A gene was disrupted. The results indicated that deletion of the LMP2A gene did not affect the immortalization efficiency of EBV in B-lymphocytes....

2015
Lukas Vrba James C Garbe Martha R Stampfer Bernard W Futscher

Immortality is an essential characteristic of human carcinoma cells. We recently developed an efficient, reproducible method that immortalizes human mammary epithelial cells (HMEC) in the absence of gross genomic changes by targeting 2 critical senescence barriers. Consistent transcriptomic changes associated with immortality were identified using microarray analysis of isogenic normal finite p...

Journal: :Molecular and cellular biology 1999
Q Gao S Srinivasan S N Boyer D E Wazer V Band

The high-risk human papillomaviruses (HPVs) are associated with carcinomas of the cervix and other genital tumors. Previous studies have identified two viral oncoproteins, E6 and E7, which are expressed in the majority of HPV-associated carcinomas. The ability of high-risk HPV E6 protein to immortalize human mammary epithelial cells (MECs) has provided a single-gene model to study the mechanism...

Journal: :Cancer research 1999
D F Jarrard S Sarkar Y Shi T R Yeager G Magrane H Kinoshita N Nassif L Meisner M A Newton F M Waldman C A Reznikoff

The cell cycle regulatory genes p16/CDKN2 and RB are frequently deleted in prostate cancers. In this study, we examined the role of alterations in p16 and pRb during growth, senescence, and immortalization in vitro of human prostate epithelial cells (HPECs). HPECs are established from normal prostate tissues and cultured on collagen-coated dishes. Our results show that p16 is reproducibly eleva...

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