Efficiently making use of granular activated carbon (GAC) to reduce pollution is always a golden goal for water industry. We developed ultrasonication collection method for determination of the biomass and microbial attachment and growth in activated carbon filter. Based on this method, we found that using hydraulic cycle to immobilize cells onto GAC can greatly improve the attachment of inocul...

A new bacteria microarray patterning technique is developed by patterning thick polycationic polymers on glass surface, which generates high-coverage and high-precision E. coli cell patterns. Cell immobilization efficiency is greatly improved, compared to conventional monolayer surface patterning approach. Cell viability tests show very low cytotoxicity of polyethyleneimine (PEI). This advancem...

2.1. Immobilization of signaling factors as a mechanism to control local concentration . . . . . . . . . . . . . 3 2.2. Sequential factors to program stem cell differentiation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4 2.3. Factors secreted or presented from differentiated cells within the niche . . . . . . . . . . . . . . . . . . . . . . 4 3. Direct cell-cell con...

In this work, the effect of immobilization of bread yeast (Saccharomyces cerevisiae) by sol-gel technique combined with functionalized nanoporous silica was investigated in different weight ratios of silica containing materials (TMOS: LUS-1). The activities of immobilized yeast in days after immobilization were examined. The results showed immobilization maintain the yeast life for a longer tim...

Case Description- Five lions (one female and 4 male) were presented for diagnostic andsurgical treatment (wound management, castration and radiography). Immobilization oranesthesia was necessary to complete the procedure.Treatment and Outcome - Immobilization was induced using intramuscular injection ofvarious drug combinations (xylazine, diazepam or midazolam in combination withketamine). Inha...

We present two microfluidic approaches for immobilizing the roundworm C. elegans on-chip. The first approach creates a CO(2) micro-environment while the second one utilizes a deformable PDMS membrane to mechanically restrict the worm's movement. An on-chip 'behavior' module was used to characterize the effect of these methods on the worm's locomotion pattern. Our results indicate that both meth...

In this work, we report a new method to reversibly immobilize proteins to a surface in a functionally active orientation directly from cell lysate by employing a fusion protein consisting of a thermal-responsive elastin (ELP) domain as the surface anchor and a calcium-responsive calmodulin (CalM) domain for protein capturing. Incorporation of an M13 tag into recombinant proteins enables not onl...

Magnetic silica aerogel in hydrophobic and hydrophilic forms were used as support to immobilize Candida rugosa lipase by adsorption method. Response surface methodology (RSM) was employed to study the effects of the three most important immobilization parameters, namely enzyme/support ratio (0.3-0.5, w/w), immobilization time (60-120 min) and alcohol percentage (20-40, %v/v) on the specific act...

The process of electrostatic extrusion as a method for cell immobilization was investigated that could be used for potential applications in medicine. An attempt was made to assess the effects of cell addition and polymer concentration on the overall entrapment procedure, ie, on each stage of immobilization: polymer-cell suspension rheological characteristics, electrostatic extrusion process, a...

Enzymatic mode of biodiesel production has recently gained a special attention among researchers because it eliminates the disadvantages of alkaline process such as end product recovery, waste water treatment, energy intensive and etc. Recently, considerable attention has paid in the direct use of intracellular lipase as a whole-cell biocatalyst (i.e immobilization of the enzyme secreting micro...