نتایج جستجو برای: cell elisa

تعداد نتایج: 1715061  

Abstract : Foot and Mouth Disease is one of  the important live stocks contagious viral disease caused by Aphtovirus genus ,  that is belong to family RNA virus  picornaviride. The important characteristic of FMD virus is high mutation that give rise to diversity of Antigen in surface of Neutralizing proteins. For this reason FMD virus have 7 distinct serotype and many subtype. Vaccination is o...

Journal: :Applied and environmental microbiology 2003
Yang Hong Mark E Berrang Tongrui Liu Charles L Hofacre Susan Sanchez Lihua Wang John J Maurer

Contamination of retail poultry by Campylobacter spp. and Salmonella enterica is a significant source of human diarrheal disease. Isolation and identification of these microorganisms require a series of biochemical and serological tests. In this study, Campylobacter ceuE and Salmonella invA genes were used to design probes in PCR-enzyme-linked immunosorbent assay (ELISA), as an alternative to c...

Journal: :Journal of Immunological Methods 2010

Journal: :Japanese journal of infectious diseases 2015
Caroline Wasonga Shingo Inoue James Kimotho Kouichi Morita Juliette Ongus Rosemary Sang Lillian Musila

Chikungunya (CHIK) is a mosquito-borne viral disease. In the 2004 CHIK outbreak in Kenya, diagnosis was delayed because of the lack of accurate diagnostics. Therefore, this study aimed to develop and evaluate an in-house IgM-capture enzyme linked immunosorbent assay (ELISA) (in-house ELISA) for the detection of chikungunya virus (CHIKV) infections. Anti-CHIKV antibodies were raised in rabbits, ...

Journal: :medical journal of islamic republic of iran 0
m raiani from the department of immunology shariati hospital, tehran university of medical sciences, tehran, islamic republic of iran. a massoud from the department of immunology shariati hospital, tehran university of medical sciences, tehran, islamic republic of iran. f davatchi from the department of rheumatology, shariati hospital, tehran university of medical sciences, tehran, islamic republic of iran.

it has been shown that activated t-cells produce and release both il-2 and il- 2 receptors (ll-2r). the rate of il-2 release is proportional to its cell surface expression and state of cell activation. to clarify the molecular basis of this immunological aberration, we analysed the amount of soluble il-2r(sil2-r) by an elisa technique in 68 patients with behcet's disease (bd), 28 patient c...

Journal: :jundishapur journal of natural pharmaceutical products 0
maryam moradi binabaj biochemistry and metabolic disorders research center, golestan university of medical sciences, gorgan, ir iran seyed ahmad hosseini nutrition and metabolic disease research center, ahvaz jundishapur university of medical sciences, ahvaz, ir iran alireza khoshbin khoshnazar biochemistry and metabolic disorders research center, golestan university of medical sciences, gorgan, ir iran jahanbakhsh asadi biochemistry and metabolic disorders research center, golestan university of medical sciences, gorgan, ir iran; biochemistry and metabolic disorders research center, golestan university of medical sciences, gorgan, ir iran. tel: +98-1732440225, fax: +98-1732421651

conclusions the increasing cell toxicity, apoptosis-inducing effects and decreasing telomerase activity may play an important role in the valproic acid and radiation mechanism. the current survey suggested that it is likely beneficial to combine valproic acid and gamma radiation to treat breast cancer. results combination of valproic acid and gamma radiation increased significantly cell toxicit...

Journal: :The new microbiologica 2007
Candan Cicek Tuba Karatas Imre Altuglu Guldane Koturoglu Zafer Kurugol Altinay Bilgic

The aim of the study was to compare an enzyme immunoassay method with shell vial cell culture method for detection of rotavirus in fecal specimens. In addition, the correlation between laboratory results and clinical scores of patients with gastroenteritis was evaluated. A total of 219 fecal specimens from children (ages 3 weeks to 5 years) with acute gastroenteritis submitted to pediatric emer...

Journal: :Analytical chemistry 2010
Kuo-Hsiang Chuang Shey-Cherng Tzou Ta-Chun Cheng Chien-Han Kao Wei-Lung Tseng Jentaie Shiea Kuang-Wen Liao Yun-Ming Wang Ya-Chen Chang Bo-Jyun Huang Chang-Jer Wu Pei-Yu Chu Steve R Roffler Tian-Lu Cheng

Poly(ethylene glycol) (PEG) is increasingly used in clinical and experimental medicine. However, quantification of PEG and PEGylated small molecules remains laborious and unsatisfactory. In this report, we stably expressed a functional anti-PEG antibody on the surface of BALB 3T3 cells (3T3/alphaPEG cells) to develop a competitive enzyme-linked immunosorbent assay (ELISA) for PEG quantification...

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