Control over spatial distributions and patterns of individual neurons their neurites provides an essential tool for studying the meaning neuronal network patterns. Moreover, complete direction control synaptic connections between cells in each is also to investigate detailed information on relationship forward feedback signaling among cells. Here, we have developed a method topographical within...

Microalgae are cultivated in macro-scale reactors traditionally and the relevant knowledge is based on bulk analysis. Whether the knowledge and laws are true for cells under micro-cultivation is still unknown. To better understand microalgal physiology, micro-cultivation of microalgae, and unicellular tracking and analysis of its response in vivo is necessary. In the study, cellular responses o...

Background For industrial pharmaceutical protein production fast growing, high producing and robust cell lines are required. To select more pH shift permissive and fast growing sub-populations, the CHO DP-12 (ATCC clone #1934) cell line, an anti-IL8 antibody producing CHO K1 (DHFR) clone, was continuously subcultured at high viability (>90 %) for more than four hundred days in shaking flasks us...

Control over spatial distribution of individual neurons and the pattern of neural network provides an important tool for studying information processing pathways during neural network formation. Moreover, the knowledge of the direction of synaptic connections between cells in each neural network can provide detailed information on the relationship between the forward and feedback signaling. We ...

Brain cells from embryonic rats were dissociated with trypsin, cultivated under constant conditions in Falcon flasks, and studied for periods of one year or more. Antisera against glial fibrillary acidic protein (GFA) and myelin basic protein (MBP) were used to identify glial cell types. For scanning electron microscopical (SEM) observation an embedding method in resin was developed that allows...

BACKGROUND Probiotic bacteria are becoming an important tool for improving human health, controlling diseases and enhancing immune responses. The availability of a cost effective cultivation conditions has profound effect on the efficiency and role of probiotic bacteria. Therefore the current study was conducted with an objective to develop a low cost growth medium for enhancing the biomass pro...

Process conditions for the synthesis and subsequent secretion of ectoine and hydroxyectoine were optimized in a cascade of two continuously operated bioreactors. In the first bioreactor the halophile bacterium Chromohalobacter salexigens was grown under continuous hyperosmotic and thermophilic conditions and therefore under constant stress driving the cells to accumulate large amounts of ectoin...

This article describes the optimization of cultivation factor settings, that is the shaking rate and working volume in 50 mL spin tubes for a Chinese hamster ovary cell line expressing recombinant human alpha-erythropoietin, using a response D-optimal surface method. The main objectives of the research were, firstly, to determine a setting in which the product titer and product quality attribut...

Embryonic Stem (ES) cells-derived cardiomyocytes can possibly be applied for cell therapy of diseases such as heart failure. Biodegradable scaffolds will significantly improve the expansion of sufficient functional ES cell-derived cardiomyocytes and may also increase the survival rate of cardiomyocytes after their transplantation. In the present study, we cultivated cardiomyocytes isolated from...

Experimental systems using non-growing cell of Eremothecium ashbyii to elucidate the mechanism of flavinogenesis were established in this paper. 1. It was found that vacuum infiltration of purines for 2, 3 and 5min and moderate shaking of non-growing cell brought about a good reproducibility of the stimulatory effects on flavinogenesis by purines and furthermore, a higher flavinogenesis. 2. The...