Alcohol sensitivity is reported in up to 74-86.5% of patients with chronic rhinosinusitis nasal polyposis (CRSwNP) and especially those aspirin-exacerbated respiratory disease (AERD). These typically report beverages fermented wood casks, fruit skins, or hops, but not other alcohol-containing drinks. We speculated that alcohol AERD/CRSwNP could result from activation basophils by polyphenols de...

Targeted therapy treatment in Colorectal Cancer (CRC) works specifically on a protein or target cell cancer. It plays role the survival of cancer cells. involves several monoclonal antibodies, one which is cetuximab targets Epidermal Growth Factor Receptor (EGFR). However, there are cases resistance caused by activities Tissue Inhibitor Metalloproteinase-1 (TIMP-1). TIMP-1 can activate downstre...

Extracellular vesicles (EVs) can modulate microenvironments by transferring biomolecules including RNAs and proteins derived from releasing cells to target cells. To understand the molecular mechanisms maintaining the neural stem cell (NSC) niche through EVs, a new transgenic (Tg) rat strain that can release human CD63-GFP expressed EVs from the NSCs was established. Human CD63-GFP expression w...

Chlamydiae are obligate intracellular bacterial pathogens that replicate solely within the confines of a membrane-bound vacuole termed an inclusion. Within this protected organelle, chlamydiae acquire host-cell-derived biosynthetic precursors necessary for intracellular subsistence, yet the mechanisms and pathways responsible for this acquisition remain elusive. The present study identifies an ...

Dear Editor, both the paper by Pereira Santos et al and the Editorial on the latest issue of this Journal, stressed on the essential, fairly irreplaceable role of basophil activation test (BAT) in allergy diagnosis (1,2). Certainly, BAT is a cell-based test and in this sense it should give fundamental insights about the role of these circulating leukocytes in hypersensitivity reactions, chronic...

PRAME (PReferentially expressed Antigen in MElanoma) is a protein highly melanoma cells and has long been thought to be nuclear protein; however, our data suggests secreted by via extracellular vesicles (EVs). Using three human cell lines vitro, we found not only detectable the intracellular location (nuclear cytoplasmic fractions), it also EVs Western blot. We used differential centrifugation ...

BACKGROUND Basophils are circulating cells involved in hypersensitivity reactions and allergy but many aspects of their activation, including the sensitivity to external triggering factors and the molecular aspects of cell responses, are still to be focused. In this context, polychromatic flow cytometry (PFC) is a proper tool to investigate basophil function, as it allows to distinguish the exp...

BACKGROUND Platelet-derived microparticles (PMPs) are generally considered a marker of platelet activation in cardiovascular disease. We studied the extent to which PMP subpopulations parallel platelet activation in vitro and in vivo. METHODS Using flow cytometry, we analyzed PMP subpopulations from resting and activated platelets in vitro (n = 6) as well as from plasma samples of patients wi...