Capillary electrophoresis (CE) techniques have been fundamental in advancing technology for the separation and identification of peptide and protein mixtures [1,2]. Today, they have become a staple in conjunction with mass spectrometry systems to resolve, identify and analyze protein and peptide characteristics with sensitivity and accuracy. Recent advances have miniaturized traditional CE inst...

Capillary zone electrophoresis was used in the analysis of anions and cations in electroless plating bath samples. Examples described in this application note include the analysis of inorganic and organic anions in nickel-plating baths, cations in nickel-iron-cobalt-plating baths and metal-EDTA complexes in plating bath waste. Sample preparation was minimal, consisting merely of diluting the sa...

Cellobiohydrolase (CBH) is an important enzyme for the conversion of lignocellulosic biomass to ethanol. This work separated the glycoforms of CBH possessing different numbers of neutral mannoses using capillary zone electrophoresis (CZE) in a 50 mM, pH 7.5 phosphate buffer. The method analysed CBH in an intact form using a polyacrylamide coated fused silica capillary without requiring additive...

Capillary zone electrophoresis was employed for the determination of histamine using end-column amperometric detection with a carbon fiber microelectrode, at a constant potential. The optimum conditions of separation and detection were 10 mmol/L phosphate buffer, pH 5.6 for the buffer solution, 15 kV for the separation voltage, and 1.35 V (versus SCE) for the detection potential. The linear ran...

We introduce the open source parameter estimation tool awkt. It is primarily aimed at assisting in the characterization and identification of globular proteins using capillary zone electrophoresis. The program untangles a proteins’ charge-to-size ratio by applying nonlinear least-squares optimization to a particular closed-form solution of the Nernst-Planck equation. Numerical estimates for the...

A simple and rapid capillary zone electrophoresis determination method with UV detection of grepafloxacin and clinafloxacin has been developed. The separation was performed in 35 mM borate-35 mM phosphate buffer solution (pH 8.6), containing 6% (v/v) of acetonitrile. Analyses were realised using fused-silica capillaries (57 cm length x 75 microm I.D.) and the operating conditions were: 15 kV ap...

A method is developed for measuring the concentration of micafungin, an echinocandin antifungal agent, in serum, using capillary zone electrophoresis. With a 0.1M borate buffer (pH 10.0) as the run buffer, detection is carried out at 200 nm. Pretreatment is performed by adding acetonitrile to serum (1:2) for deproteinization, allowing the supernatant fluid to be taken for measurement. The detec...

Synthesis and characterization of a polymethine cyanine near-infrared (NIR) fluorophoric label for the derivatization and determination of fatty acids separated by capillary electrophoresis are described. The label contains an aromatic amine functionality, which is used to form a covalent linkage with the analyte. Various linking chemistries are explored, including direct amine–acid condensatio...