Here we describe some of the crucial steps to generate induced pluripotent stem cells (iPSCs) using mRNA transfection. Our approach uses a V. virus-derived capping enzyme instead of a cap-analog, ensuring 100% proper cap orientation for in vitro transcribed mRNA. V. virus' 2'-O-Methyltransferase enzyme creates a cap1 structure found in higher eukaryotes and has higher translation efficiency com...

Algal biorefinery process utilizes every component of algal biomass to produce multiple useful fuel products. In this technique, acid pretreatment of algal biomass hydrolyzes microalgal carbohydrates into fermentable sugars, makes lipids more extractable and a protein part accessible for additional products. In the present study, Chlorella sorkiniana produced higher quantity of biodiesel than B...

p. atlantica subsp. kurdica, with the local name of baneh, is a wild medicinal plant which grows in kurdistan, iran.  the identification of resistance gene analogs holds great promise for the development of resistant cultivars. a pcr approach with degenerate primers designed according to conserved nbs-lrr (nucleotide binding site-leucine rich repeat) regions of known disease-resistance (r) gene...

0.279 0.490 0.029 BLEU 0.338 0.469 0.086 cap-macro approx 0.340 0.676 0.086 approx+cap-micro and BLEU 0.354 0.734 0.086 cap-micro approx 0.406 0.741 0.086 cap-micro orig 0.413 0.769 0.086 cap-micro tagger 0.413 0.769 0.086 cap-micro approx-restr 0.423 0.800 0.143 cap-macro orig 0.428 0.800 0.143 cap-macro tagger 0.608 0.800 0.400 cap-macro approx-restr Avg Max Min Overlapping Approximation Czec...

the aim of this work was to investigate the influence of interactions between modified starch (hi-cap 100) and the nonionic surfactant tween 80 on the properties of cardamom oil-in-water emulsion and its corresponding spray dried microcapsules. to this end the mean diameter and size distribution of droplets as well as their polydispersity index and also the viscosity and flow behavior of emulsi...

twenty-one patients with advanced breast cancer (7 premenopausal and 14 postmenopausal women) were treated with a combination of cyclophosphamide, doxorubicin, and cisplatin (cap). the median age of the patients was 43 years (range 36-61). this therapy was repeated every 3 weeks. nine patients (group 1) received cap as primary therapy for metastatic breast cancer, and twelve patients (group 2) ...