Calf diarrhea (scours) is a primary cause of illness and death in young calves. Significant economic losses associated with this disease include morbidity, mortality, and direct cost of treatment. Multiple pathogens are responsible for infectious diarrhea, including, but not limited to, Bovine coronavirus (BCV), bovine Rotavirus A (BRV), and Cryptosporidium spp. Identification and isolation of ...

The cellular uptake of PMOs (phosphorodiamidate morpholino oligomers) can be enhanced by their conjugation to arginine-rich CPPs (cell-penetrating peptides). Here, we discuss our recent findings regarding (R-Ahx-R)(4)AhxB (Ahx is 6-aminohexanoic acid and B is beta-alanine) CPP-PMO conjugates in DMD (Duchenne muscular dystrophy) and murine coronavirus research. An (R-Ahx-R)(4)AhxB-PMO conjugate ...

A one-tube reverse transcription-polymerase chain reaction (RT-PCR) for absolute feline coronavirus (FCoV) quantitation was developed. The assay is based on the 5' nuclease activity of the Thermus flavus (Tfl) polymerase and a fluorogenic probe which generates fluorescence when it is cleaved. The fluorogenic probe, also called TaqMan(TM) probe (Perkin Elmer, Foster City, USA), is an oligonucleo...

Non-structural Proteins 7 and 8 of Feline Coronavirus Form 1 a 2:1 Heterotrimer that Exhibits Primer-independent RNA 2 Polymerase Activity 3 4 Yibei Xiao†, Qingjun Ma†, Tobias Restle, Weifeng Shang, Dmitri I. 5 Svergun, Rajesh Ponnusamy, Georg Sczakiel, Rolf Hilgenfeld* 6 7 Institute of Biochemistry, Institute of Molecular Medicine, Center for 8 Structural and Cell Biology in Medicine, Universi...

| Adaptation is often thought to affect the likelihood that a virus will be able to successfully emerge in a new host species. If so, surveillance for genetic markers of adaptation could help to predict the risk of disease emergence. However, adaptation is difficult to distinguish conclusively from the other processes that generate genetic change. In this Review we survey the research on the ho...

a 1:1 matched case-control study of calves under 1 month of age was carried out by weekly visits to 7 dairy farms in mashhad from may 2008 to october 2008. fecal samples were collected from a total of 112 calves with clinical signs of diarrhea and from 112 matched animals without clinical signs of diarrhea as assessed by a scoring system. the samples were investigated for the presence of rotavi...