Esterification and transesterification of high free fatty acid (FFA) oils to fatty acid alkyl ester (FAAE) by sol-gel entrapped lipase B from Candida antarctica (CalB) and lipase from Burkholderia cepacia were investigated. The optimal conditions for the esterification catalyzed by the immobilized CalB were determined to be 50 °C, 1.7:1 molar ratio of methanol to fatty acid, and 0.36 g of water...

A general chemo-enzymatic approach to synthesize both enantioenriched trans-3-alkoxyamino-4-oxy-2-piperidones, which are important scaffold for various naturally occurring alkaloids, is reported. To this end, a selective transition-metal-free dual C−H oxidation of piperidines mediated by the TEMPO oxoammonium cation (TEMPO+) was used, followed enzymatic resolution corresponding alkoxyamino-2-pi...

In this paper, we describe the effectiveness of combination between an organic solvent system mixture with orthoformates different chain sizes from one to four carbon atoms. These orthoesters have been used as a “water trapper/alcohol releaser molecule” reach notable improvement in enantioselectivity and enantiomeric excess our target compound, (S)-2-(4-isobutylphenyl)propanoic acid (ibuprofen ...

The kinetics of alcoholysis of methyl propionate and n-propanol catalyzed by Candida antarctica lipase B supported onto silanized Chromosorb P was studied in a continuous solid/gas reactor. In this system the solid phase is composed of a packed enzymatic sample and is percolated by nitrogen as carrier gas, which simultaneously carries substrates to the enzyme while removing reaction products. I...

Interest in the synthesis and application of thymol esters has increased recent years due to numerous applications associated with its biological activities. The enzymatic octanoate by esterification octanoic acid was explored using soluble lipases immobilized lipase biocatalysts solvent-free systems. Candida antarctica B form most active biocatalyst for this reaction. Different feeding strateg...

We herein report the first directed evolution of Candida antarctica lipase A (CalA), employing a combinatorial active-site saturation test (CAST). Wild-type CalA has a modest E-value of 5.1 in kinetic resolution of 4-nitrophenyl 2-methylheptanoate. Enzyme variants were expressed in Pichia pastoris by using the episomal vector pBGP1 which allowed efficient secretory expression of the lipase. Ite...

The main monomer of tomato cuticle, 10,16-dihydroxyhexadecanoic acid (10,16-DHPA) and its methyl ester derivative (methyl-10,16-dihydroxyhexadecanote; methyl-10,16-DHHD), were used to study their oligomerization reactions catalyzed by five lipases: Candida antarctica lipase B (CAL-B), Rhizomucor miehei lipase (RM), Thermomyces lanuginosus lipase (TL), Pseudomonas cepacia lipase (PCL) and porcin...

A lipase-based assay for detection of specific DNA sequences has been developed. Lipase from Candida antarctica was conjugated to DNA and captured on magnetic beads in a sandwich assay, in which the binding was dependent on the presence of a specific target DNA. For amplification and to generate a detectable readout the captured lipase was applied to an optical assay that takes advantage of the...