To determine the proangiogenesis effect of series of saccharides and a synthetic oligosaccharide and potential mechanisms, an in vitro 3-dimensional endothelial cell sprouting (3D-ECS) assay and the chick chorioallantoic membrane (CAM) model were used. We demonstrated that a sulfated oligosaccharide significantly promotes the endothelial capillary network initiated by vascular endothelial growt...

35S-Labeled calmodulin (CaM) was used to screen a tobacco anther cDNA library. A positive clone (NtER1) with high homology to an early ethylene-up-regulated gene (ER66) in tomato, and an Arabidopsis homolog was isolated and characterized. Based on the helical wheel projection, a 25-mer peptide corresponding to the predicted CaM-binding region of NtER1 (amino acids 796-820) was synthesized. The ...

A mitogen-activated protein kinase (MAPK) phosphatase gene, designated NtMKP1, was isolated as a candidate gene for a calmodulin (CaM)-binding protein from tobacco. NtMKP1 protein has four characteristic domains conserved among plant MAPK phosphatases reported so far, namely a dual specificity protein phosphatase catalytic domain, gelsolin-like domain, putative CaM-binding domain (CaMBD), and s...

The receptor kinase BRI1 (BRASSINOSTEROID-INSENSITIVE 1) is a key component in BR (brassinosteroid) perception and signal transduction, and has a broad impact on plant growth and development. In the present study, we demonstrate that Arabidopsis CaM (calmodulin) binds to the recombinant cytoplasmic domain of BRI1 in a Ca2+-dependent manner in vitro. In silico analysis predicted binding to Helix...

complementary and alternative medicine (cam) is widely used by patients worldwide. financial factors may influence the decision to use cam. national health systems are requested to consider cam in their health plans.

This study represents a global survey of the times of the first appearance of the neuron-glia cell adhesion molecule (Ng-CAM) in various regions and on particular cells of the chick embryonic nervous system. Ng-CAM, originally characterized by means of an in vitro binding assay between glial cells and brain membrane vesicles, first appears in development at the surface of early postmitotic neur...

Objective: To examine the antiangiogenic effect of some flavonoids (taxifolin and phloretin) against various In vivo and In vitro experimental models such as Chick Chorio Allantoic Membrane (CAM) assay, Dorsal Skinfold Chamber (DSFC) and Tube formation assay. Methods: Three different concentrations of drug solution (40μM, 80 μM, 120 μM) and (50 μM, 100 μM, 150 μM) were applied topically during ...

Today's medical world has gained a growing appeal towards CAM/TM which can be seen in the strategies of US in the form of establishing National Center for Complementary and Alternative Medicine (NCCAM) at the National Institutes of Health (NIH) and White House Commission on Complementary and Alternative Medicine Policy (WHCCAMP) at the White House, the annual increase of integration of CAM clas...

Today's medical world has gained a growing appeal towards CAM/TM which can be seen in the strategies of US in the form of establishing National Center for Complementary and Alternative Medicine (NCCAM) at the National Institutes of Health (NIH) and White House Commission on Complementary and Alternative Medicine Policy (WHCCAMP) at the White House, the annual increase of integration of CAM clas...

BACKGROUND Platelet-rich fibrin (PRF), a platelet-rich plasma (PRP) derivative mainly composed of fibrin networks, has been increasingly demonstrated to be effective in wound healing in clinical and pre-clinical animal studies. However, there has still been a concern that major growth factors may significantly be loss from PRF during its preparation through the slow clotting process. To address...