Acetylcholine was identified and quantified by g.l.c. in moss callus. The acetylcholine concentrations in the moss callus were found to be regulated by phytochrome. The highest concentrations of acetylcholine occurred after irradiation with red light. Moss callus grown in the dark contained no acetylcholine.

Callus formation occurs in the presence of secondary bone healing and has relevance to the fracture's mechanical environment. An objective image processing algorithm was developed to standardize the quantitative measurement of periosteal callus area in plain radiographs of long bone fractures. Algorithm accuracy and sensitivity were evaluated using surrogate models. For algorithm validation, ca...

in order to optimize callus induction in pistacia atlantica desf. subsp. kurdica rech. f., the effect of different plant growth regulator (pgr) combinations were studied. inflorescence axis explants were cultured on wpm medium supplemented with 1, 2 and 3 mg/l of tdz or 1, 2 and 3 mg/l of ba, in combination with 1mg/l different auxins: 2, 4-d,  naa  and iba . callus initiation was observed in a...

In this study, we prepared and characterized a callus extract from Citrus junos and assessed its utility as a source of topical anti-aging ingredients. Callus extract was produced by aqueous extraction from Citrus junos grown on Murashige and Skoog medium with picloram as a growth regulator. After measuring the total phenolic and flavonoid contents, the major phenolic compound in calli was iden...

Callus-forming capacity is enhanced with hypocotyl maturity in Arabidopsis. However, the genetic regulation of age-related gain in capacity for callus formation is unclear. We used a gene expression microarray assay to characterize the underlying mechanisms during callus formation in young and mature hypocotyl explants of Arabidopsis. As expected, genes involved in photosynthesis and cell wall ...

BACKGROUND Tea is an economic important crop with high medicinal value due to rich polyphenols content. In the present research we studied the accumulation of polyphenols of in vitro regenerated callus from anthers. OBJECTIVE Callus induction of tea anthers and in vitro accumulation of phenolic compounds from the anther-derived callus. MATERIALS AND METHODS Standardization of callus inducti...

The development of somatic cells in to embryogenic cells occurs in several stages and ends in somatic embryo formation, though most of these biochemical and molecular changes have yet to be elucidated. Somatic embryogenesis coupled with genetic transformation could be a biotechnological tool to improve potential crop yields potential in sugarcane cultivars. The objective of this study was to ob...

The present study was carried out to build up an efficient protocol for various concentrations of 2, 4D for “Callus formation and somatic embryogenesis in sugarcane (Saccharum spp L.) using various concentrations of 2, 4-D and RAPD analysis of regenerated plants in the laboratory of Biotechnology Nuclear Institute of Agriculture, Tandojam during the year 2015 to 2016. Three sugarcane varieties ...

Many surgeons assess the biological activity of fracture nonunions by the presence or absence of callus on radiographs. However, the assessment of biological activity by radiographic appearance alone is controversial. Bone scintigraphy reflects blood flow and new bone formation; therefore, it is useful in assessing such biological activity in nonunion cases. This retrospective study compared ra...

In five cultivated rice varieties e.g., Nonabokra, PNR 381, Taipei 309, Koshika and TKM 11 the response in the initiation of callus and its size varied greatly among varieties, salt concentrations (NaCl and Na2SO4) and their interaction. Better regeneration ability of Nonabokra, Koshika and TKM 11 was found to be correlated to small size callus with a high concentration of Na2SO4. The regenerat...