We previously showed that the solution to the primal problem is equivalent to the solution to the dual problem if they satisfy the following primal-dual equivalence conditions. First, we need a convex objective function and in our case, it is 1 2 ||~ w||2. Second, we need convex inequality constraints gi, which are 1−yi(~ w · ~xi + b) for i = 1, ...,m. The last condition states that for each in...

Isolation and taxonomic and phylogenetic studies performed in this decade have revealed that most Gram-positive, aerobic spore-forming bacteria which have halophilic/halotolerant/alkaliphilic and/or alkalitolerant properties compose a considerably large phylogenetic group within rRNA 1 group of Ash et al. (1991) in the phyletic assemblage of bacteria classically defined as the genus Bacillus (A...

Abstract Introduction Calmodulin (CaM) is a highly conserved mediator of calcium (Ca2+) dependent signalling. Its flexible structure allows CaM to bind and modulate many targets, including cardiac ion channels. Genotyping has revealed several mutations associated with congenital disorders heart rhythm, known as long QT-syndrome (LQTS). LQTS patients suffer from prolonged ventricular recovery ti...

Psittacine beak and feather disease virus (PBFDV) and avian polyomavirus (APV) are the most common viral diseases in psittacine birds, both affecting feathers and physical appearance of birds. Between 2005 and 2009, a total of 269 samples were collected from birds presented at veterinary clinics, shelters and rescue centers of wildlife in Costa Rica. They belonged to 19 species of psittacine bi...

TOPIC: Lung Cancer TYPE: Original Investigations PURPOSE: Non-small cell lung cancer (NSCLC), one of the leading causes cancer-related death, has a low 5-year survival rate owing to inevitable acquired resistance toward antitumor drugs, platinum-based chemotherapy, and targeted therapy. Epidermal growth factor (EGF)-EGF receptor (EGFR) signaling activates downstream events phospholipase C/inosi...

N-Formimino-l-glutamate iminohydrolase (HutF) from Pseudomonas aeruginosa catalyzes the deimination of N-formimino-l-glutamate in the histidine degradation pathway. An amino acid sequence alignment between HutF and members of the amidohydrolase superfamily containing mononuclear metal centers indicated that residues Glu-235, His-269, and Asp-320 are involved in substrate binding and activation ...

We developed a method to evaluate the activity of Na+–Ca2+ exchanger (NCX) and sarco-endoplasmic reticulum Ca2+-ATPase (SERCA) with fluorescence microscopy in mouse ventricular cardiomyocytes. In non-beating cardiomyocytes, α-adrenoceptor stimulation by phenylephrine caused decrease cytoplasmic Ca2+ concentration, which was inhibited SEA0400, an NCX inhibitor, but not cyclopiazonic acid, SERCA ...

Protein phosphatase-1 (PP1) is expressed ubiquitously and is involved in many eukaryotic cellular functions, although PP1 enzyme activity could not be detected in the social amoeba Dictyostelium discoideum cell extracts. In the present paper, we show that D. discoideum has a single copy gene that codes for the catalytic subunit of PP1 (DdPP1c). DdPP1c is expressed throughout the D. discoideum l...

We report for the first time on the rapid X-ray variability of the galactic bulge source and X-ray burster SLX 1735–269. The power spectrum as observed with the Rossi X-ray Timing Explorer is characterized by a strong band-limited noise component which is approximately flat below a 0.1–2.3 Hz break frequency; above this frequency the power spectrum declines as a power law of index 0.9. At the h...

The conserved residues His-162 and Arg-269 of the flavoprotein p-hydroxybenzoate hydroxylase (EC 1.14.13.2) are located at the entrance of the interdomain cleft that leads toward the active site. To study their putative role in NADPH binding, His-162 and Arg-269 were selectively changed by site-specific mutagenesis. The catalytic properties of H162R, H162Y, and R269K were similar to the wild-ty...