A single form of tRNA(Trp) exists in the yeast cytoplasm to respond to the unique codon, UGG, which specifies this amino acid. Mutations in the anticodon of the corresponding gene, which generate potential nonsense suppressor tRNAs, have been generated in vitro and tested in vivo for biological activity. The amber (C35U) and opal (C34U) suppressors show strong and weak activities respectively w...

A gel shift assay that distinguishes the aminoacylated form from the deacylated form of tRNAs was used to study the requirements for aminoacylation of Escherichia coli tRNA(Asn) in vivo. tRNA(Asn) derivatives containing single base changes in their anticodons or discriminator bases were constructed, and the extent of in vivo aminoacylation was determined directly. Substitution of U35 with C35 o...

Myosin null mutants of Dictyostelium are defective for cytokinesis, multicellular development, and capping of surface proteins. We have used these cells as transformation recipients for an altered myosin heavy chain gene that encodes a protein bearing a carboxy-terminal 34-kD truncation. This truncation eliminates threonine phosphorylation sites previously shown to control filament assembly in ...

In this geostatistical analysis we present the results of interrelation between unemployment rate and lung cancer incidence ratios in the Province of Opole, Poland. In the study, unemployment statistics and population data were analyzed together with the registered (histopathologically confirmed) lung cancer cases (C34, ICD10) in sex-stratified working age population (18-65 years). The data wer...

The Wage Premium of Foreign Education: New Evidence from Australia We study whether Australian employers recognise immigrants’ education acquired abroad, and if so how. Using data from the Longitudinal Surveys of Immigrants in Australia, we apply interval regression to model migrant hourly earnings. We find substantially higher returns from human capital obtained in Australia and other OECD cou...

Estimates of the Effect of Parents’ Schooling on Children’s Schooling Using Censored and Uncensored Samples In this paper we estimate the impact of parental schooling on child schooling, focus on the problem that children who are still in school constitute censored observations, and evaluate three solutions to it: maximum likelihood approach, replacement of observed with expected years of schoo...

The remediation of petroleum hydrocarbons (TPH) in a contaminated soil by electrokinetic (EK) treatment was studied the laboratory. effects applying constant electrical current on pH, moisture content, conductivity (EC), temperature, and concentrations three fractions TPH (C10–C16, C17–C34 C35–C40) were investigated. experiment run for seven days samples collected at end 7 day period analysis p...