Clostridium perfringens is a gram-positive, anaerobic bacterium that causes a wide range of diseases in humans and animals. Th e purposes of this study were to determine, using the enzyme-linked immunosorbent assay (ELISA), the clostridial toxins in intestinal samples of lambs with suspected enterotoxemia; to conduct molecular typing of C. perfringens isolates; and to investigate the presence o...

BACKGROUND Control of brucellosis in livestock, wildlife and humans depends on the reliability of the methods used for detection and identification of bacteria. In the present study, we describe the evaluation of the recently established real-time PCR assay based on the Brucella-specific insertion sequence IS711 with blood samples from 199 wild boars (first group of animals) and tissue samples ...

We evaluated a commercially produced enzyme-linked immunosorbent assay (ELISA; LMD Laboratories, Inc.) for the detection of Cryptosporidium spp. in 296 stool specimens submitted to the Mayo Clinic parasitology laboratory for routine examination. The specimens examined were fresh (4 specimens), were stored frozen at -65 degrees C (49 specimens), or were preserved in 10% formalin (243 specimens)....

Monoclonal antibody (MAb)-based sandwich direct enzyme-linked immunosorbent assay (MSD-ELISA) methods that can detect foot-and-mouth disease virus (FMDV) antigens, both multiserotype (MSD-ELISA/MS) (for O, A, C, and Asia 1) and single-serotype (MSD-ELISA/SS) (for O, A, and Asia 1, specifically), were developed. MAb 1H5 was used as an antigen-trapping antibody that reacted with all seven serotyp...

Synthetic peptide antigens were prepared for use in enzyme-linked immunosorbent assays (ELISAs) to detect serum antibodies against abortigenic strains of Chlamydia psittaci in livestock. Peptide antigens were identified with C. psittaci B577-immune sera by solid-phase scanning of overlapping octapeptides of variable domains (VDs) of the major outer membrane protein of C. psittaci serovar 1 (omp...

The prevalence of Helicobacter pylori infection in a population-based sample of 477 children (mean age plus minus standard deviation, 5.8 plus minus 0.5 years) determined by the [(13)C]urea breath test ([(13)C]UBT) was 10.7% (95% confidence interval [CI], 8.1 to 13.8%), and that determined by salivary enzyme-linked immunosorbent assay (ELISA) was 11.9% (95% CI, 9.2 to 15.2%). Compared to the [(...

To assess the importance of only IgA antibody positivity in the peptide-based ELISA (P-ELISA) examination of kinetic behaviors of antibodies (IgA, IgG) to Chlamydia trachomatis, 426 sera from 52 follow-up antigen-positive patients were assayed. In part, a microimmunofluorescence (MIF) test and an immunoblot (IB) assay were also used for confirmation. The results showed that the positivity rates...

The performance of a commercial immunofluorescence assay (IFA commercial), an in-house in-house) and indirect enzyme-linked immunosorbent (ELISA) were evaluated in the detection antibodies anti-C. burnetii serum Q fever patients persons without disease. For study, seropositive seronegative samples for (n = 200) from bank Instituto Adolfo Lutz Brazil used. Commercial IFA was considered this stud...

fast enzyme linked immunosorbent assay (fast-elisa) was compared with the standard elisa for the diagnosis of human hydatidosis. seventy serum samples including 30 from hydatidosis patients (surgically confirmed), healthy control individuals not infected with any parasitic diseases (n=/20) and from others with different parasitic infections including, toxocariosis (n=5), fasciolosis (n=5), tric...