نتایج جستجو برای: but in nested pcr reaction
تعداد نتایج: 17135550 فیلتر نتایج به سال:
126 abstract: in this research we studied the effects of cyclic hydration and dehydration of cysts of artemia urmiana and artemia parthenogenetica on the hatching percentage, survival and growth. the experiment was carried out in 3 treatments (1-3 hydration/dehydration cycles) with 3 replicates for each treatment. later effects of cold preservation at -20°c during three time periods were ...
تعیین گونه های عامل لیشمانیوز پوستی به روش مولکولی pcr-nested و rflpدرشهرستان جهرم. لیشمانیوز یکی از بیماریهای انگلی می باشد که توسط تک باخته ای از جنس لیشمانیا ایجاد می شود. این بیماری به صورت گسترده در مناطق مختلفی از جهان مشاهده می شود . بیماری دارای طیف وسیعی بوده که در یک طرف آن زخمی ساده است و در طرف دیگر طیف اشکال پوستی ، پوستی مخاطی ، و لیشمانیوز احشایی می باشد که حتی می تواند منجر به ...
abstract in first part of this project, the use of a new and biguanid-like catalyst supported on silica as a recyclable catalyst provides a new route for the synthesis of a variety of arylalkylidene rhodanine derivatives through knoevenagle reaction in at present of solvent at room temperature. rhodanine derivatives and especially arylalkylidene rhodanines have proven to be attractive compound...
SARAVANAN, M., M. PARTHIBAN, P. RAMADASS: Genotyping of rotavirus of neonatal calves by nested-multiplex PCR in India. Vet. arhiv 76, 497-505, 2006. ABSTRACT A total of 209 diarrhoeic faecal samples were collected from calves in nine diary cattle herds located in different areas in Tamil Nadu state, India. Nine faecal samples positive for group A rotavirus by polyacrylamide gel electrophoresis ...
introduction: breast cancers in the early phase frequently undergo distant metastasis and survival of patients is greatly dependent on distant metastasis. the occurrence of micrometastasis has been suggested to relate with prognostic features of breast cancer, such as lymph node metastasis and the presence of vascular invasion. the aim of this study was to examine the presence of keratin-19 mrn...
Screening blood donations for anti-HCV antibodies and alanine aminotransferase (ALT) serum levels generally prevents the transmission of hepatitis C virus (HCV) by transfusion. The aim of the present study was to evaluate the efficiency of the enzyme immunoassay (EIA) screening policy in identifying potentially infectious blood donors capable to transmit hepatitis C through blood transfusion. W...
BACKGROUND Dengue fever results from infection with one or more of four different serotypes of dengue virus (DENV). Despite the widespread nature of this infection, available molecular diagnostics have significant limitations. The aim of this study was to develop a multiplex, real-time, reverse transcriptase-PCR (rRT-PCR) for the detection, quantitation, and serotyping of dengue viruses in a si...
objective: the objective of this research was to determine the prevalence of genital c. trachomatis infection in asymptomatic women by using highly sensitive nested-polymerase chain reaction (pcr) in urine sample. materials and methods: one hundred-forty asymptomatic women were randomly selected from those who attended gynecology out patient department of hazraat e rasool hospital in tehran. fi...
The polymerase chain reaction (PCR) and its variations, such as the nested-PCR, have been described as promising techniques for rapid diagnosis of tuberculosis (TB). With the aim of evaluating the usefulness of a nested-PCR method on samples of blood and urine of patients suspected of tuberculosis we analyzed 192 clinical samples, using as a molecular target the insertion element IS6110 specifi...
The clinical value of an in-house cytomegalovirus nested polymerase chain reaction (CMV-PCR) and a commercial molecular assay hybrid capture CMV DNA assay (HCA) was evaluated in monitoring a group of renal transplant patients for six months follow up. In this study, the sensitivity, specificity, positive predictive value, and negative predictive value of nested CMV DNA PCR assay and HCA at the ...
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