the major aim of this study was to compare the morphology and activity of rumen protozoa of khuzestan water buffalo and holstein cow using in vitro digestibility and gas production parameters of steam treated sugarcane pith. rumen fluid obtained from two buffalo and cow steers fed the same diet, 30:70 concentrate: forage. to separate rumen protozoa, antibiotic solution and fungicides were added...

Effect of co-culture of buffalo preantral follicles with different somatic cells, i.e. cumulus cells, granulosa cells, Ovarian Mesenchymal Cells (OMC) and Oviductal Epithelial Cells (OEC) was studied. Large preantral follicles were isolated by microdissecting the trypsin (1%) digested ovarian cortical slices. Cumulus cells were isolated by repeated pipetting of oocytes, granulosa cells were iso...

Assessing roles of people, technology and structure in emergency management systems: a public sector perspective Minkyun Kim a Now at Department of Business Management and Economics, College of Social Science, Dongduk Women's University, Seoul, Korea. , Raj Sharman b , Catherine P. Cook-Cottone c , H. Raghav Rao b & Shambhu J. Upadhyaya d a Department of Operations Management and Strategy, Scho...

Following fertilization, the early embryo is transcriptionally silent and early development is directed by the complement of maternally-inherited mRNAs and proteins. At some point, however, a maternal to embryonic transition occurs, in which further development is directed by the zygotic transcripts. So to determine this transition stage, the quantitative expression pattern of HMGN-2 and CREB g...

There are morphological and reproductive physiological differences between swamp buffalo (Bubaluscarabanensis) and river buffalo (Bubalus bubalis). The development of fetus weight and fetus biometry wasreported in river buffalo and other animals but not in swamp buffalo. The aim of this study was to describethe inherent variability in fetus related measurements during swamp buffalo pregnancy. T...

The present study was designed to test the efficacy, as assessed by blastocyst production, of culturing slaughterhouse-derived bovine oocytes in Ménézo's B2 (B2) medium with or without serum and with or without buffalo rat cell (BRL) coculture. In addition, OPU-derived oocytes were cultured in B2-BRL coculture with or without serum for the first 72 h or TCM 199-BRL coculture with serum and the ...

The study aimed to determine the effects of different concentrations of glycerol and ethylene glycol and ultrarapid cryopreservation (vitrification) on survival and subsequent in vitro development of bubaline cumulus oocyte complexes (COCs) in order to recognize the optimum cryoprotectant. Survival and in vitro maturation, fertilization and cleavage of buffalo COCs was evaluated subsequent to t...