enterococci are gram positive and catalase- negative cocci that are found in the gastrointestinal tract of mammals and birds, and are readily isolated from soil, surface and waters. the aim of this study was to discriminate between enterococcus isolates based on repetitive element sequence based –pcr (rep-pcr) with the boxa2r primer and their antibiotics profile. enterococci isolates were obtai...

Routine procedures used to isolate Haemophilus ducreyi in a busy laboratory are reported. Identification was based on colony morphology and nutritional and biochemical properties of 120 fresh isolates of H. ducreyi. These isolates grew very well on Gonococcal Agar and Mueller-Hinton Agar incubated at 34 degrees C in candle extinction jars containing moistened filter paper. Colonies varied in si...

Contamination sites of Pseudomonas fluorescenswere traced in the production line for milk pasteurization in a large dairy plant in Shiraz, Iran. Samples of raw and pasteurized milk were collected at six sites along the line. All milk samples were incubated at 7°C until the aerobic plate count had reached 10 6 -10 7 cfu mL-1. Colonies were picked randomly and identified. No growth of gram negati...

Anthracnose caused by Colletotrichum species is a common postharvest disease of banana fruit. We investigated and identified Colletotrichum species associated with anthracnose in several local banana cultivars based on morphological characteristics and sequencing of ITS regions and of the β-tubulin gene. Thirty-eight Colletotrichum isolates were encountered in anthracnose lesions of five local ...

Diatoms of Gomishan wetland (Golestan province, N. Iran), one of the most significant habitats in the eastern shore of the Caspian Sea, has been isolated, purified and identified during 2013. Using serial dilution and streaking on F/2 medium, pure, monoalgal and axenic cultures of the isolates were prepared. The isolates were characterized and identified using micro-morphological studies on the...

The anthracnose disease caused by Colletotrichum species is well-known as a major plant pathogen that primarily causes fruit rot in pepper and reduces its marketability. Thirty-five isolates representing species of Colletotrichum were obtained from chili fruits showing anthracnose disease symptoms in Chungcheongnam-do and Chungcheongbuk-do, South Korea. These 35 isolates were characterized acco...

background: lower respiratory tract infections (ltris) are among the most common infectious diseases with potential life-threatening complications. methods: the study consisted of 426 patients with suspected ltris from mid and far western region of nepal between september 2011 and july 2014. the specimens were collected and processed according to the standard microbiological methods at the cent...

bacterial resistance to antibiotics is a serious problem and is increasing in prevalence world-wide at an alarming rate. the antimicrobial susceptibility patterns of 1897 gram-positive bacterial isolates were evaluated. the minimum inhibitory concentration (mic) of isolates which comprised staphylococcus aureus (927 isolates), coagulase-negative staphylococci (cns; 425 isolates), enterococcus f...