Sulfur mustard (SM) is a strong alkylating and mutagenic compound that targets humanairway system. We considered the expression of Forkhead box M1 (FOXM1) and apolipoproteinE (APOE) genes, which are responsible for cell proliferation, differentiation, tumorigenesis,and increased risk of lung cancer, in the lung bronchial tissue of patients exposed to SM.After performing pulmonary functional tes...

We have generated 47 DNA pools and 235 subpools from 21,049 T-DNA insertion lines of rice. DNA pools of 500-1,000 lines were adequate for screening a T-DNA insertion within a 2-kb region. To examine the efficacy of the DNA pools, we selected MADS-box genes, which play an important role in controlling various aspects of plant development. A total of 34 MIKC-type MADS-box genes have now been iden...

In mammals, sexual differentiation of the gonads during development depends on the presence of the Y chromosome (1). The testis-determining gene lies on the Y chromosome, and has been delimited in human to a 35-kilobase region near the pseudoautosomal boundary (2). SRY is a gene located in this region and both conserved during evolution and Y chromosome specific in a number of mammals (3). The ...

In Brazil, the common bean is a crop with significant social and economic importance. The prospecting of N2 fixing bacteria crucial since biological nitrogen fixation (BNF) an eco-friendly technique. This work aimed to obtain characterize rhizobium isolates based on morpho-physiological, molecular, symbiotic efficiency parameters, using strains SEMIA 4077, 4080, 4088 as references. characterist...

The cycling parameters for BOX A1R PCR were modified and the primer-specific annealing temperature was optimized. Using Trout Lake Escherichia coli isolates, four different template preparation methods were compared. The cell washing, heat lysis, and DNA isolation methods all gave clear and bright band patterns with good reproducibility. The cell washing template preparation method for direct u...

We characterized indigenous common bean rhizobia from five districts of the state of Minas Gerais, Brazil. The isolates were trapped by two common bean varieties, the Mineiro Precoce (Andean origin) and Ouro Negro (Mesoamerican origin). Analysis by BOX-PCR of selected isolates detected a high level of genetic diversity.

Abbreviations: AG, anaplastic glioma; BCNU, 1,3-bis( CCNU, 1-(2-chloroethyl)-3-cyclohexyl-1-nitrosourea; G ratio; O-meG, O-methylguanine; MGMT, O-methylgu MSP,methylation-specific PCR; PFS, progression-free surv temozolomide ⁎ Corresponding author at: Department of Neurol Washington, Box 356470, 1959 NE Pacific Street, Se Fax: +1 206 543 8315. E-mail address: [email protected] (J.R. Sil...

BACKGROUND The endometrium prepares for implantation under the control of steroid hormones. It has been suggested that there are complicated interactions between the epithelium and stroma in the endometrium during menstrual cycle. In this study, we demonstrate a difference in gene expression between the epithelial and stromal areas of the secretory human endometrium using microdissection and ma...

The diversity of Ochrobactrum anthropi, Ochrobactrum intermedium, Ochrobactrum tritici and Ochrobactrum grignonense in agricultural soil and on the wheat rhizoplane was investigated. O. anthropi was isolated both from soil and from the rhizoplane, O. intermedium and grignonense only from bulk soil, and O. tritici only from the wheat rhizoplane. On the genetic level, the immunotrapped isolates a...