The existence of an intermediate in the biosynthesis of serum albumin was suggested by a previous study of the kinetics of appearance of Cl4 in soluble serum albumin in liver slices (1). In the course of investigations on the nature of this intermediate, it has been found that treatment of cytoplasmic particles of chicken liver with deoxycholate or other agents will release non-dialyzable mater...

T HE SEPARATION of morphologically distinct cells by differences in their specific gravity has been demonstrated using density gradient centrifugation in several support media. From bovine serum albumin density gradient centrifugation experiments, morphologically similar lymphocytes have been shown to consist of several closely spaced, discontinuous, discrete populations.5 In addition, erythroc...

The anionic calixarenes para-sulphonatocalix[4]arene and 1,3-di-O-phosphonatocalix[ 4]arene, have been used to cap silver nanoparticles. The binding of these functional particles with regard to various serum albumins (bovine serum albumin, human serum albumin, porcine serum albumin and sheep serum albumin) has been studied by variable temperature fluorescence spectroscopy. The quenching of the ...

A nano-thick solid-like water film on solid surfaces plays an important role in various fields, including biology, materials science, atmospheric chemistry, catalysis and astrophysics. Visualising the water nanofilm has been a challenge due to its dynamic nature and nanoscale thickness. Here we report an ion diffusion method to address this problem using a membrane formed with a BSA-Na2CO3 (BSA...

The interactions between milk proteins, beta-Lactoglobulin (beta-Lg) and bovine serum albumin (BSA), at the air-water interface have been evaluated. The surface pressure (pi), molecular area (a) isotherms were obtained by compression of the monolayers at different pH and temperature. In the method used to calculate the interactions, the desorbed segments of the proteins into the aqueous subphas...

Sustained-release systems were made by incorporating bovine serum albumin and magnetic steel beads in an ethylene-vinyl acetate copolymer matrix. When exposed to aqueous medium, the polymer matrix released the albumin slowly and continuously. Application of an oscillating magnetic field increased the release rate by as much as 100%. Intervals of 6-hr periods of magnetic exposure and nonexposure...

Long, nonseptate filamentous cells consisting of 5 to 40 single-cell unit lengths were formed from Escherichia coli surface mutant ONT-3 by treatment with a sublethal concentration of sodium dodecyl sylfate. As distinct from several other elongated cells (e.g., thymine-starved filaments), it was found here that stable giant spheroplasts, 5 to 10 micrometers in diameter, were produced by the act...

Determination of microgram quantities of protein in the Bradford Coomassie brilliant blue assay is accomplished by measurement of absorbance at 590 nm. This most common assay enables rapid and simple protein quantification in cell lysates, cellular fractions, or recombinant protein samples, for the purpose of normalization of biochemical measurements. However, an intrinsic nonlinearity compromi...

The experimental results of a previous study of the mass transfer kinetics of bovine serum albumin (BSA) in ion-exchange chromatography under nonlinear conditions are reevaluated. The analysis of the concentration dependence of the lumped mass-transfer rate coefficient (k(m,L)) provides information on the kinetics of axial dispersion, fluid-to-particle mass transfer, intraparticle mass transfer...