Proteolysis of bovine serum albumin by trypsin.

the purpose of this study was to determine the prevalence of bovine leukemia virus (blv) in khorasan razavi and khorasan shomali provinces which are the main provinces located in the northeast of iran. total number of 429 blood samples were collected from industrial dairy herds. the samples were categorized based on province, age (2-3, 4-6, and 7-10 years old), calving (≤ 2, 3-5, and > 5) and h...

bovine ephemeral fever (bef) is an acute epidemic disease in cattle and water buffalo, spanning tropical and subtropical zones of asia, australia, and africa. in recent years, bef has been distributed in many provinces of iran and caused economic losses. in this study the sequence encoding the g1 epitope of bovine ephemeral fever virus (befv) g glycoprotein was amplified by pcr, ligated into th...

three new rimantadine schiff bases (rsbs) were prepared, and then the interaction of rsbs with bovine serum albumin (bsa) was investigated using fluorescence, synchronous fluorescence, uv-vis absorption spectroscopy under physiological conditions. the results showed that the three rsbs effectively quenched the intrinsic fluorescence of bsa via static quenching. binding constant (ka), number of ...

the aim of the present study was to find the effects of pre and post vitrification taxol treatment on bovineimmature cumulus oocyte complexes (cocs). bovine ovaries were collected from the local slaughterhousein a container with 30-37°c physiologic saline and supplemented with antibiotics. the grade a aspiratedcocs were assigned to 6 experimental groups; the 1st group was control (n=100) in whi...

the objective of this study was to compare an indirect elisa, based on a purified 60 kda envelope glycoprotein (gp51su), with a pourquire indirect elisa for the detection of antibodies to the bovine leukemia virus. for conducting this research, 340 serum samples were collected from two different breeds of cows (sarabi and holestin) in different herds. commercial elisa revealed positive results ...

the measurement of antibody responses of animals exposed to bvdv either through a natural exposure or an immunization protocol is still a standard procedure. for bvdv, the test formats have been largely limited to elisa which is a valuable diagnostic test to measure the level of bvdv specific antibodies as well as antigen in blood samples. in the present study, 120 blood samples were collected ...

The antiviral effects of bovine interferons on the replication of bovine respiratory tract viruses were studied. Bovine turbinate monolayer cultures were treated with bovine interferons and challenged with several bovine herpesvirus 1 strains, bovine viral diarrhea virus, parainfluenza type 3 virus, goat respiratory syncytial virus, bovine respiratory syncytial virus, bovine adenovirus type 7, ...

Gastric aspartic proteases (AP) are among proteolytic enzymes that are widely distributed in vertebrates. They are responsible for the digestion of dietary proteins and are synthesized as inactive precursors (prochymosin, pepsinogen and progastricsin), commonly known as zymogens. One part of their secretion by mucous neck and chief cells in the gastric mucosa (2, 22, 23) reaches the blood strea...

Two pepsinogens (pepsinogens 1 and 2) were purified from the esophageal mucosa of the bullfrog (Rana catesbeiana), and their molecular weights were determined to be 40,100 and 39,200, respectively, by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. The NH2-terminal 70-residue sequences of both pepsinogens are the same, including the 36-residue activation segment. Furthermore, a cDNA ...