abstract background and aim: increased rate of micronucleus in buccal mucosa cells and its correlation with carcinogenesis is worth consideration. recently, occupation in dental laboratories has been proposed as a predisposing factor. therefore, the present study aimed to evaluate the effect of occupational exposure on buccal mucosa cells of dental laboratory technicians. materials and methods:...

Cytochalasine B is an indicator in the CBMN assay, but can also be geno- or cytotoxic. The aim of study was to determine optimal dose cytochalasine for Cytokinesis-Block Micronucleus (CBMN) assay domestic cat. results revealed a surprisingly high number micronuclei (MN) relation doses used. It found that concentration 2.5 μg/ml enough obtain binuclear cells, with minimal toxic effect on lymphoc...

Both selenium (Se) and polysaccharides from Pyracantha fortuneana (Maxim.) Li (PFPs) (P. fortuneana) have been reported to possess antioxidative and immuno-protective activities. Whether or not Se-containing polysaccharides (Se-PFPs) have synergistic effect of Se and polysaccharides on enhancing the antioxidant and immune activities remains to be determined. We previously reported that polysacc...

stem cells are of great interest for regenerating disturbed tissues and organs. these cells are commonly isolated from the bone marrow, but there has been interest in other tissues in the recent years. in this study, we evaluated the possibility of isolation of stem cells from oral connective tissue and investigated their characteristics.in this experimental study, sampling from the bone marrow...

Chemical mutagenicity is a major hazard that is important to workers' health. Despite the use of large amounts of allyl chloride, the available mutagenicity data for this chemical remains controversial. To clarify the mutagenicity of allyl chloride and because a micronucleus (MN) test had not yet been conducted, we screened for MN induction by using male ICR mice bone marrow cells. The test res...

Most cells deficient in 3-methyladenine (3MeA) DNA glycosylase become sensitive to the lethal and clastogenic effects of alkylating agents. Surprisingly, myeloid progenitor bone marrow (BM) cells derived from Aag -/- mice were more resistant than those from wild-type mice to the cytotoxic effects of several alkylating agents. Moreover, an alkylation-resistant phenotype was observed in vivo usin...

Radioprotective effectiveness of a combination of HT and AET was evaluated by radiation induced micronucleus screening in mouse bone marrow. Swiss albino mice were injected with 2-Aminoethylisothiouronium bromide hydrobromide (AET)+5-hydroxy-L-tryptophan (HT) 30 minutes prior to a single whole body exposure of 8 and 12 Gy Co60 gamma rays. Animals were sacrificed at different intervals viz., 24 ...

Erythrocyte-based micronucleus tests have traditionally analyzed bone marrow because splenic filtration in most species removes micronucleated cells from peripheral blood. We have evaluated a flow cytometric method for monitoring micronucleated reticulocyte frequencies (%MN-RET) in the peripheral blood of beagle dogs treated with cyclophosphamide (CP) and have found that analysis of micronuclea...