The aim of this study was to explore the characteristics blaCTX-M-27 carriage and mobilization in Salmonella Escherichia coli isolates from food-producing animals China. A total 2280 E. 229 collected food June 2003 September 2014 were screened for presence gene. blaCTX-M-27-positive typed plasmid DNA sequenced determine genetic context types present. Bacterial fitness evaluated by growth curve ...

OBJECTIVE To investigate the local epidemiology of Klebsiella penumoniae carrying bla(CTX-M-15) in southern China and to characterize the genetic environment of bla(CTX-M-15). METHODS PCR and DNA sequencing were used to detect and characterize the genetic contexts of bla(CTX-M-15). The clonal relatedness of isolates carrying bla(CTX-M-15) was determined by pulse-field gel electrophoresis. Con...

conclusions our study emphasized the increasing role of esbl genes, especially blactx-m15 in epec isolates. results of 42 epec, eight isolates carried the blactx-m1. none of the isolates carried blactx-m2 and blactx-m9. the blactx-m15 variant was identified in all of blactx-m1-positive isolates. furthermore, blashv and blatem genes were detected in 40.5% (n = 17) and 19% (n = 8) of all epec iso...

AIM To determine the prevalence and genotypic characterisation of extended spectrum beta-lactamases produced by gram negative bacilli isolated at Mbarara Regional Referral Hospital (MRRH). SAMPLES Gram negative clinical isolates. STUDY DESIGN Laboratory-based descriptive cross-sectional study. PLACE AND DURATION OF THE STUDY MRRH, June and August 2012. METHODS Gram negative clinical iso...

AGGAATCCCATG-30 and CTX-M-R2, 50-GCCGTCTAAGGCGATAAAC-30, were used to amplify the entire allele (876 bp) for sequencing. This revealed that S. enterica serovar Senftenberg strain 9-4 produced CTX-M-3, which was first identified in Poland. Southern hybridization using the whole blaCTX-M gene as a probe showed that the gene was located on a plasmid of < 38 kb in size. Attempts to transfer this pl...

The aim of this work was to investigate the epidemiological and genetic characteristics of ESBL-producing Klebsiella pneumoniae (ESBL-Kp) causing community-onset infections. K. pneumoniae isolates were collected from 31 Chinese secondary hospitals between August 2010 and 2011. Genes encoding ESBL and AmpC beta-lactamases were detected by PCR. The isolates were assigned to sequence types (STs) u...