For regulated secretion, mast cells and several other cell types utilize compound exocytosis, a combination of granule-plasma membrane and granule-granule fusions. The molecular machinery that controls this massive export process has not been identified. We report that SNAP-23, a t-SNARE related to SNAP-25, relocates in response to stimulation from plasma membrane lamellipodia-like projections ...

Granule cell dispersion is a characteristic feature of Ammon's horn sclerosis in temporal lobe epilepsy. It was recently shown that granule cell dispersion is associated with decreased expression of the extracellular matrix protein Reelin. Reelin controls neuronal lamination and the differentiation of dendrites and spines. Here, we studied dendritic orientation and the distribution of dendritic...

• Mathematical model of drug release from drug-excipient granules. Developed can account for radial variations in porosity and content. Model accounts internal diffusion solubility-limited extraction granule. Solubility-limited give rise to a range moving boundary value problems. A reduced-order is presented the case which initial load greatly exceeds solubility Understanding pharmaceutical gra...

The objective of this study was to determine the density anaerobic granules on different heights a full-scale Upflow Anaerobic Sludge Bed reactor. defined through settling velocities granules, measured in In study, granular calculated with and developed mathematical model. model is based Stokes experiment, were taken from an reactor, 0.6 7.6 m. granules’ diameters varied between 1 5 mm. six vel...

Normal-mode observations of seven quiet regions, obtained by the Hinode spacecraft, are used to analyze the physical properties of granules with mean upward and downward Doppler velocity. We identify 75 146 granules from the observations with a granuledetection method. Then the granules are divided into two subsets: one with negative mean Doppler velocity (granule-upflows), and the other with p...

Aberrant reorganization of dentate granule cell axons, the mossy fibers, occurs in human temporal lobe epilepsy and rodent epilepsy models. Whether this plasticity results from the remodeling of preexisting mossy fibers or instead reflects an abnormality of developing dentate granule cells is unknown. Because these neurons continue to be generated in the adult rodent and their production increa...

Type I lissencephaly, a neuronal migration disorder characterized by cognitive disability and refractory epilepsy, is often caused by heterozygous mutations in the LIS1 gene. Histopathologies of malformation-associated epilepsies have been well described, but it remains unclear whether hyperexcitability is attributable to disruptions in neuronal organization or abnormal circuit function. Here, ...

During development of the cerebellum, Sonic hedgehog (SHH) is expressed in migrating and settled Purkinje neurons and is directly responsible for proliferation of granule cell precursors in the external germinal layer. We have previously demonstrated that SHH interacts with vitronectin in the differentiation of spinal motor neurons. Here, we analysed whether similar interactions between SHH and...