Rhizobium rhizogenes-mediated plant hairy root induction is a convenient method for functional study of root-specific genes. To develop an optimized tobacco system and gene function in nicotine biosynthesis, we investigated by three R. rhizogenes strains, R1601, K599, LBA9402, on different media with leaf discs from plants ages, observed that the strain LBA9402 used explant infection exhibited ...