magnetic nanoparticles attracted a great deal of attention in the medical applications due to their unique properties. the most exceptional property of magnetic particles is their response to a magnetic force, and this property has been utilized in applications such as drug targeting, bioseparation, contrast agents in magnetic resonance imaging (mri) and heating mediators for cancer therapy. in...

Low-cost protein purification methods are in high demand for mass production of low-selling price enzymes that play an important role in the upcoming bioeconomy. A simple protein purification method was developed based on affinity adsorption of a cellulose-binding module-tagged protein on regenerated amorphous cellulose (RAC) followed by modest desorption. The biodegradable cellulosic adsorbent...

Proof of concept for the in vivo bacterial production of a polyester resin displaying various customizable affinity protein binding domains is provided. This was achieved by engineering various protein binding domains into a bacterial polyester-synthesizing enzyme. Affinity binding domains based on various structural folds and derived from molecular libraries were used to demonstrate the potent...

Recent progress in the development and production of new, innovative protein therapeutics require rapid adjustable high-resolution bioseparation techniques. Sodium dodecyl sulfate capillary gel electrophoresis (SDS-CGE) using a borate (B) cross-linked dextran (D) separation matrix is widely employed today for consistency analysis therapeutic proteins manufacturing release testing. Transient cro...

This paper deals with the purification of four proteins from Euphorbia characias latex, a copper amine oxidase, a nucleotide pyrophosphatase/phosphodiesterase, a peroxidase, and a purple acid phosphatase. These proteins, very different in molecular weight, in primary structure, and in the catalyzed reaction, are purified using identical preliminary steps of purification and by chromatographic m...

Water contamination by heavy metal pollution is induced rapid industrialization and urbanization. Removal of ions from wastewater prime importance for a clean environment human health. The problem seriously threatened to In addition, these metals are toxic carcinogenic. They cause serious problems aquatic ecosystems especially humans. Different methods have been utilized remove the wastewater, ...

BACKGROUND Avidin is a chicken egg-white protein with high affinity to vitamin H, also known as D-biotin. Many applications in life science research are based on this strong interaction. Avidin is a homotetrameric protein, which promotes its modification to symmetrical entities. Dual-chain avidin, a genetically engineered avidin form, has two circularly permuted chicken avidin monomers that are...

Cryogel beads, fabricated by the microchannel liquid-flow focusing and cryo-polymerization method, have micron-scale supermacropores allowing the passage of crude feedstocks, and could be of interest as chromatographic adsorbents in bioseparation applications. In this work, we provide a rapid freezing and continuous formation method for cryogel beads by cryo-polymerization using dry ice particl...

Figure 1. Flowchart of the fabrication process for interconnected silica nanotubes from hyperbranched PbSe nanowires. Inorganic nanofluidic devices, such as nanopores, nanochannels, and nanotubes (NTs) have been actively studied in bioseparation, bioanalysis, fluidic transistors, power generation, and fast mass transport. Compared to biological nanopores, inorganic nanofluidic devices have been...

Biomaterials are being used for the healthcare applications from ancient times. But subsequent evolution has made them more versatile and has increased their utility. Biomaterials have revolutionized the areas like bioengineering and tissue engineering for the development of novel strategies to combat life threatening diseases. Together with biomaterials, stem cell technology is also being used...