Staphylococcus aureus (S. aureus), which has developed multidrug resistance, leads to many healthcare-associated infections resulting in significant medical and economic losses. Therefore, the development of new efficient strategies deal with these bacteria been gaining importance. Lysostaphin is a peptidoglycan hydrolase that considerable potential as bacteriocin. However, there have few repor...

−Bacterial cellulose (BC) was produced by Acetobacter xylinum KJ1 in a modified airlift-type bubble column bioreactor, which had a low shear stress and high oxygen transfer rate (kLa). Saccharified food wastes (SFW) were used as the BC production medium due to its low cost. An aeration rate of 1.2 vvm (6 L/min) was tentatively determined as the optimal aeration condition in a 10 L spherical typ...

Cellular therapies are emerging as a standard approach for the treatment of several diseases. However, realizing the promise of cellular therapies across the full range of treatable disorders will require large-scale, controlled, reproducible culture methods. Bioreactor systems offer the scale-up and monitoring needed, but standard stirred bioreactor cultures do not allow for the real-time regu...

Monoclonal antibody production in commercial scale cell culture bioprocessing requires a thorough understanding of the engineering process and components used throughout manufacturing. It is important to identify high impact components early on during the lifecycle of a biotechnology-derived product. While cell culture media selection is of obvious importance to the health and productivity of m...

A MULTI-SCALE APPROACH TO FED-BATCH BIOREACTOR CONTROL Abhishek Soni , M.S. University of Pittsburgh The rising energy costs, increased global competition in terms of both price and quality, and the need to make products in an environmentally benign manner have paved the way for the biological route towards manufacturing. Many of the products obtained by the biological route either cannot be pr...

BACKGROUND Upstream bioprocesses are extremely complex since living organisms are used to generate active pharmaceutical ingredients (APIs). Cells in culture behave uniquely in response to their environment, thus culture conditions must be precisely defined and controlled in order for productivity and product quality to be reproducible. Thus, development culturing platforms are needed where man...

A novel milliliter-scale bioreactor equipped with a gas-inducing impeller was developed with oxygen transfer coefficients as high as in laboratory and industrial stirred-tank bioreactors. The bioreactor reaches oxygen transfer coefficients of >0.4 s(-1). Oxygen transfer coefficients of >0.2 s(-1) can be maintained over a range of 8- to 12-mL reaction volume. A reaction block with integrated hea...

Bacillus subtilis is a non-pathogenic, sporulating, gram-positive bacteria with pronounced antimicrobial and metabolic activity great potential for wide application in various fields. The aim of this paper was to determine the optimum B. NCIM 2063 growth conditions scale up biomass production from shake flasks bioreactor level. critical parameters their interaction effects were studied using Bo...

This paper begins by providing an outline of various waste stabilisation techniques on which the concept of bioreactor or process-based landfills is based, followed by an overview of the research and development of this alternative landfill approach. The paper then presents a summary of the Australian experience. It also reports on the current state of bioreactor landfills and discusses the dif...