MLAA-34 is a newly identified monocytic leukemia-associated antigen that is overexpressed in acute monocytic leukemia specifically, thus providing a novel target for the therapy of acute monocytic leukemia. In this study, we first expressed MLAA-34 protein in Escherichia coli (E.coli) BL21 (DE3) cells and purified it by nickel ion affinity chromatography with high purity (>90%). Then, MLAA-34 w...

The aim of this study was to identify potential ligands and develop a novel diagnostic test to pathogenic bovine rotavirus (BRV) using phage display technology. The viruses were used as an immobilized target followed by incubation with a 12-mer phage display random peptide library. After five rounds of biopanning, phages had a specific binding activity to BRV were isolated. DNA sequencing indic...

Epithelial cell adhesion molecule (EpCAM) plays an important role in tumorigenesis. Camelids produce functional antibodies composed of heavy chains only that bind to their antigens via a single domain variable fragment known as nanobody. Nanobodies show multiple advantages over traditional monoclonal antibodies. Isolation anti-EpCAM nanobodies (Nbs) was the main aim this study. An immune nanobo...

Mycophenolic acid (MPA) is an immunosuppressant drug commonly used to prevent organ rejection in transplanted patients. MPA monitoring of great interest due its small therapeutic window. In this work, a phage-displayed peptide library was select cyclic peptides that bind the MPA-specific recombinant antibody fragment (Fab) and mimic behavior MPA. After biopanning, several were isolated tested c...

Cow milk is an important source of food protein for children, however, it could lead to allergy, especially infant. α-lactalbumin (α-LA) and β-lactoglobulin (β-LG) from whey make up a relatively high proportion proteins have received widespread attention as major allergens in milk. However, few studies identified the epitopes both simultaneously. In this study, ImmunoCAP indirect ELISA were fir...